Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GSU80_RS16700 Genome accession   NZ_CP047231
Coordinates   3436742..3438238 (-) Length   498 a.a.
NCBI ID   WP_001040664.1    Uniprot ID   -
Organism   Escherichia coli strain BL21-AI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3431742..3443238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSU80_RS16675 (GSU80_16675) panM 3431965..3432348 (+) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
  GSU80_RS16680 (GSU80_16680) livJ 3432547..3433650 (-) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GSU80_RS16685 (GSU80_16685) rpoH 3433921..3434775 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GSU80_RS16690 (GSU80_16690) ftsX 3435020..3436078 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  GSU80_RS16695 (GSU80_16695) ftsE 3436071..3436739 (-) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  GSU80_RS16700 (GSU80_16700) pilA 3436742..3438238 (-) 1497 WP_001040664.1 signal recognition particle-docking protein FtsY Machinery gene
  GSU80_RS16705 (GSU80_16705) rsmD 3438388..3438984 (+) 597 WP_000743204.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GSU80_RS16710 (GSU80_16710) yhhL 3438974..3439243 (+) 270 WP_001295207.1 DUF1145 family protein -
  GSU80_RS16715 (GSU80_16715) yhhM 3439246..3439605 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  GSU80_RS16720 (GSU80_16720) yhhN 3439746..3440372 (+) 627 WP_000964718.1 lysoplasmalogenase -
  GSU80_RS16725 (GSU80_16725) zntA 3440446..3442644 (+) 2199 WP_000106517.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GSU80_RS16730 (GSU80_16730) tusA 3442746..3442991 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54698.53 Da        Isoelectric Point: 4.1560

>NTDB_id=358249 GSU80_RS16700 WP_001040664.1 3436742..3438238(-) (pilA) [Escherichia coli strain BL21-AI]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAQEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=358249 GSU80_RS16700 WP_001040664.1 3436742..3438238(-) (pilA) [Escherichia coli strain BL21-AI]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTAGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGCAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGTGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACATTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGTCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378