Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E6B08_RS26690 Genome accession   NZ_CP039371
Coordinates   5736633..5738000 (-) Length   455 a.a.
NCBI ID   WP_136916706.1    Uniprot ID   -
Organism   Pseudomonas putida strain 1290     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5731633..5743000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6B08_RS26670 (E6B08_26670) - 5731927..5732124 (-) 198 WP_008094130.1 YbdD/YjiX family protein -
  E6B08_RS26675 (E6B08_26675) - 5732138..5734204 (-) 2067 WP_136916703.1 carbon starvation CstA family protein -
  E6B08_RS26680 (E6B08_26680) - 5734372..5734737 (+) 366 WP_136916704.1 PilZ domain-containing protein -
  E6B08_RS26685 (E6B08_26685) - 5734998..5736515 (+) 1518 WP_136916705.1 nucleobase:cation symporter-2 family protein -
  E6B08_RS26690 (E6B08_26690) radA 5736633..5738000 (-) 1368 WP_136916706.1 DNA repair protein RadA Machinery gene
  E6B08_RS26695 (E6B08_26695) mscL 5738178..5738597 (+) 420 WP_133332150.1 large-conductance mechanosensitive channel protein MscL -
  E6B08_RS26700 (E6B08_26700) - 5738629..5739405 (-) 777 WP_136916707.1 ferredoxin--NADP reductase -
  E6B08_RS26705 (E6B08_26705) - 5739617..5740330 (+) 714 WP_136916708.1 autoinducer binding domain-containing protein -
  E6B08_RS26710 (E6B08_26710) - 5740425..5741549 (+) 1125 WP_136916709.1 methyltransferase -
  E6B08_RS26715 (E6B08_26715) - 5741673..5741825 (-) 153 WP_136916710.1 DUF2474 domain-containing protein -
  E6B08_RS26720 (E6B08_26720) cydB 5741838..5742845 (-) 1008 WP_136916711.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48699.10 Da        Isoelectric Point: 6.8985

>NTDB_id=358229 E6B08_RS26690 WP_136916706.1 5736633..5738000(-) (radA) [Pseudomonas putida strain 1290]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETIIA
TARQEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=358229 E6B08_RS26690 WP_136916706.1 5736633..5738000(-) (radA) [Pseudomonas putida strain 1290]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAGTGCGGTGCGACCTTTCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCTTGGTCGAGACCATGATCGAGAGCGGCGGGGCCGCAGCGCCAAGCGGCCGTGCCGGTTGGACCG
GGCAGCAGGCGCAGATCAAGACCCTGGCCGAGGTCAGCGTCGAGGAAATCCCGCGTTTCACCACCAGCAGTACCGAGCTG
GATCGCGTGCTGGGCGGCGGGCTGGTGGATGGTTCGGTGGTGCTGATCGGCGGTGATCCTGGCATCGGCAAGTCGACCAT
CCTGTTGCAAACCTTGTGCAACATTGCCGTGGGCATGCCCGCGTTGTACGTCACCGGCGAAGAATCGCAACAGCAGGTGG
CCATGCGTTCGCGGCGCCTGGGGCTGCCCCAGGACCAACTCAAGGTCATGACCGAGACCTGCATCGAGACCATCATCGCC
ACTGCCCGCCAGGAAAAACCGCGGGTCATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGTTGCAGTCGGCGCC
CGGCGGCGTCGCCCAGGTGCGTGAGAGCACGGCGCTGCTGGTGCGCTATGCCAAGCAGAGCGGTACCGCGATCTTCCTAG
TCGGCCACGTCACCAAGGAGGGATCGCTGGCAGGTCCCCGGGTGCTCGAGCACATGGTCGACACCGTGCTGTATTTCGAA
GGCGAGTCCGATGGCCGCCTGCGTCTGCTGCGTGCGGTGAAGAACCGTTTTGGCGCGGTCAACGAACTGGGCGTGTTCGG
CATGACCGATCGCGGCCTCAAGGAGGTGTCGAACCCGTCGGCGATCTTCCTCAACCGCACCCAGGAAGAGGTGCCCGGCA
GCGTGGTCATGGCCACCTGGGAGGGAACCCGGCCGATGCTGGTGGAGGTGCAGGCGCTGGTCGACGACAGCCACCTGGCC
AACCCGCGGCGGGTCACCCTTGGCCTGGATCAGAACCGCCTGGCCATGCTGCTGGCGGTGCTGCACCGGCATGGCGGCAT
TCCCACGCACGACCAGGATGTATTCCTCAACGTGGTTGGCGGGGTCAAGGTGCTGGAGACCGCTTCGGACCTGGCCCTGT
TGGCTGCGGTGATGTCCAGCCTGCGCAACCGGCCATTGGCCCATGGGCTGCTGGTGTTCGGCGAAATCGGCTTGTCGGGC
GAGGTGCGGCCGGTGCCCAGTGGCCAGGAGCGGCTGAAGGAAGCGGCCAAGCATGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAGTCGCCGGCGGGGCTGCAGGTGATTGCCGTCACGCGGCTGGAACAGGCCCTGGACGCCCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(354-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.488
  radA Streptococcus pneumoniae Rx1

46.154

100

0.462
  radA Streptococcus pneumoniae D39

46.154

100

0.462
  radA Streptococcus pneumoniae R6

46.154

100

0.462
  radA Streptococcus pneumoniae TIGR4

46.154

100

0.462
  radA Streptococcus mitis SK321

46.154

100

0.462
  radA Streptococcus mitis NCTC 12261

45.934

100

0.459

Multiple sequence alignment