Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GSF12_RS06160 Genome accession   NZ_CP047226
Coordinates   1350907..1351875 (+) Length   322 a.a.
NCBI ID   WP_159374796.1    Uniprot ID   -
Organism   Moraxella osloensis strain YV1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1345907..1356875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS06140 (GSF12_06140) dusB 1346237..1347235 (-) 999 WP_159374792.1 tRNA dihydrouridine synthase DusB -
  GSF12_RS06145 (GSF12_06145) tnpA 1347449..1347859 (-) 411 WP_159374793.1 IS200/IS605 family transposase -
  GSF12_RS06150 (GSF12_06150) - 1347908..1349011 (+) 1104 WP_159374794.1 RNA-guided endonuclease InsQ/TnpB family protein -
  GSF12_RS06155 (GSF12_06155) rlmN 1349498..1350697 (+) 1200 WP_159374795.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GSF12_RS06160 (GSF12_06160) pilF 1350907..1351875 (+) 969 WP_159374796.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GSF12_RS06165 (GSF12_06165) - 1351877..1352785 (+) 909 WP_099808110.1 helix-turn-helix domain-containing protein -
  GSF12_RS06170 (GSF12_06170) ispG 1352850..1353965 (+) 1116 WP_159374797.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GSF12_RS06175 (GSF12_06175) hisS 1354013..1355311 (+) 1299 WP_159374798.1 histidine--tRNA ligase -
  GSF12_RS06180 (GSF12_06180) - 1355377..1356216 (+) 840 WP_159374799.1 YfgM family protein -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35626.71 Da        Isoelectric Point: 9.2352

>NTDB_id=358173 GSF12_RS06160 WP_159374796.1 1350907..1351875(+) (pilF) [Moraxella osloensis strain YV1]
MPPCHSRQTHLYHSQPAILCQLDDNHIEDNDKMPGKSNFLSKVFLSKIFAVGMIALSSFTLCLTGCTTTRQVTTQTVTAS
DPSRWEKNPEEIARIRTAIAAEYIRGGKLDDAKRQLESALKSYPKSAEANDMMGVLLQQEGSPRNMQKAEAYFKNAIALN
PDFSQANNNYGVYLAQLSRNQEAIAQFQIAGSALGYDGRASALENLGRTALKVKNKPLAIQSFIKALDANRQSIVARTEL
IDILLADGKFLEAQALYNDLVKIIGQSNLDPRTLSQGIRLAKLANNPLEMQKLAQQLLDRYPLSDEAKQIRSGLLTPTST
WK

Nucleotide


Download         Length: 969 bp        

>NTDB_id=358173 GSF12_RS06160 WP_159374796.1 1350907..1351875(+) (pilF) [Moraxella osloensis strain YV1]
ATGCCACCATGTCACTCACGTCAAACTCATTTATACCACAGCCAACCTGCTATTTTGTGTCAATTAGACGACAACCATAT
AGAAGACAATGATAAAATGCCGGGCAAATCAAATTTTTTATCGAAGGTTTTTTTATCTAAGATTTTTGCCGTAGGTATGA
TTGCCCTTTCAAGCTTCACTCTTTGCCTAACGGGTTGTACCACCACCCGACAAGTCACCACGCAGACAGTTACTGCCAGT
GACCCAAGTCGTTGGGAAAAAAATCCAGAAGAAATCGCCAGAATTCGCACCGCGATTGCTGCTGAATATATTCGCGGTGG
TAAATTAGACGATGCCAAACGGCAGTTAGAAAGTGCATTAAAGTCTTATCCAAAGTCAGCAGAAGCCAATGATATGATGG
GCGTGTTATTGCAGCAAGAAGGCAGCCCGCGCAATATGCAAAAAGCCGAAGCTTATTTTAAAAACGCTATCGCCCTCAAT
CCTGACTTTAGTCAAGCAAACAACAATTATGGCGTCTATCTCGCCCAACTCAGTCGCAACCAAGAAGCCATTGCCCAGTT
TCAAATTGCGGGTTCTGCCTTAGGATATGACGGTCGTGCCAGTGCGTTAGAGAATTTAGGTCGTACAGCATTAAAGGTCA
AAAACAAACCGCTGGCGATACAGTCATTTATCAAAGCGCTCGATGCCAACCGCCAAAGCATCGTCGCCCGTACAGAGCTT
ATTGATATTTTATTGGCCGATGGCAAATTTTTGGAGGCGCAGGCGCTGTATAATGATTTGGTTAAAATTATTGGTCAATC
CAACCTTGACCCGCGCACTTTATCGCAAGGGATTAGGCTTGCCAAACTTGCGAACAATCCCTTGGAAATGCAAAAATTAG
CGCAGCAGCTTTTGGATCGCTATCCACTTAGCGATGAAGCAAAGCAGATTCGTTCTGGACTTTTAACCCCAACGTCAACT
TGGAAATAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_16)

(145-189)

Tetratricopeptide repeat (TPR_16)

(100-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

41.993

87.267

0.366
  pilF Acinetobacter baylyi ADP1

41.343

87.888

0.363

Multiple sequence alignment