Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EA743_RS16855 Genome accession   NZ_CP039341
Coordinates   3467460..3468260 (-) Length   266 a.a.
NCBI ID   WP_193759251.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain TG31986     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3462460..3473260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EA743_RS16835 (EA743_016835) - 3463525..3464223 (-) 699 WP_000058788.1 YfgM family protein -
  EA743_RS16840 (EA743_016840) hisS 3464252..3465544 (-) 1293 WP_000095252.1 histidine--tRNA ligase -
  EA743_RS16845 (EA743_016845) ispG 3465541..3466656 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EA743_RS16850 (EA743_016850) - 3466678..3467469 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  EA743_RS16855 (EA743_016855) pilF 3467460..3468260 (-) 801 WP_193759251.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EA743_RS16860 (EA743_016860) rlmN 3468276..3469508 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EA743_RS16865 (EA743_016865) ndk 3469631..3470062 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EA743_RS16870 (EA743_016870) iscX 3470176..3470376 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  EA743_RS16875 (EA743_016875) - 3470476..3471027 (-) 552 WP_000669566.1 phosphatase PAP2 family protein -
  EA743_RS16880 (EA743_016880) - 3471052..3472350 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30110.44 Da        Isoelectric Point: 9.2307

>NTDB_id=358153 EA743_RS16855 WP_193759251.1 3467460..3468260(-) (pilF) [Acinetobacter baumannii strain TG31986]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=358153 EA743_RS16855 WP_193759251.1 3467460..3468260(-) (pilF) [Acinetobacter baumannii strain TG31986]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTGTTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment