Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DHCVS_RS00270 Genome accession   NC_013552
Coordinates   53427..54815 (+) Length   462 a.a.
NCBI ID   WP_012881409.1    Uniprot ID   A0A0C6EMF5
Organism   Dehalococcoides mccartyi VS     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48427..59815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DHCVS_RS00260 (DhcVS_56) - 50378..52852 (+) 2475 WP_012881407.1 ATP-dependent Clp protease ATP-binding subunit -
  DHCVS_RS00265 (DhcVS_57) - 52975..53364 (+) 390 WP_012881408.1 hypothetical protein -
  DHCVS_RS00270 (DhcVS_58) radA 53427..54815 (+) 1389 WP_012881409.1 DNA repair protein RadA Machinery gene
  DHCVS_RS00275 (DhcVS_59) - 54802..55485 (+) 684 WP_012881410.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DHCVS_RS00280 (DhcVS_60) ispF 55491..55964 (+) 474 WP_012881411.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DHCVS_RS00285 (DhcVS_61) cysS 55968..57341 (+) 1374 WP_012881412.1 cysteine--tRNA ligase -
  DHCVS_RS00295 (DhcVS_63) - 57561..58559 (+) 999 WP_012881413.1 tyrosine-type recombinase/integrase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49476.80 Da        Isoelectric Point: 7.2840

>NTDB_id=35812 DHCVS_RS00270 WP_012881409.1 53427..54815(+) (radA) [Dehalococcoides mccartyi VS]
MEKSRRVYVCSNCGRESLKWLGRCPGCQEWNTLEETTVVSPVGRKNEPARVISPAAELSSLQAAETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLSPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQTNTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGEMR
TISHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVRLALTGVKTETEDVFE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=35812 DHCVS_RS00270 WP_012881409.1 53427..54815(+) (radA) [Dehalococcoides mccartyi VS]
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCCAACTGCGGGCGTGAGAGCCTAAAGTGGCTGGGGCGCTGTCCCGGCTG
TCAGGAATGGAATACCCTTGAAGAAACAACGGTTGTTTCACCTGTAGGGCGTAAAAATGAACCTGCCCGTGTTATCAGCC
CGGCGGCTGAGCTTTCCAGTCTGCAGGCAGCCGAAACCACCCGGCGGAGTTTATCTATTTCGGAGTTCAACCGGGTATTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTGGGTGGTGAGCCAGGAATTGGCAAATCCACTCTTCTTCTTCAGGT
GGCGGCTTCGGTAGCACAAAGCGGCGGTAAGGTAGTATATGTTTCAGGGGAAGAAAACCCCGCTCAGATAAAAATGCGCG
CCCAACGCCTTGGTATCAGCGGCGAAGGGCTTTTCCTTATGGCTGAGACCGACCTTAATGCCATTCTGGCCCAGCTTTCA
GTCCTCAGCCCGTCATTGGTCGTTATTGACTCTATCCAAACTGTATTCCTGCCGGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTACGGGAATCAGCCCTCCGCCTTATGCAGTGGGCTAAAAGCAGCGGTGCCAGTGTATTTATAACAGCCCATG
TTACCAAAGAAGGCAATATAGCCGGGCCGCGCATATTGGAACACATAGTAGATGTAGTCATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTAGTGCGTTCTGTCAAAAACCGCTTCGGTTCCACTAACGAAGTAGGTATATTTGAAATGAAAAG
TGAAGGTCTGGTAGAAGTAGCTAACCCTTCCCAGATATTTTTATCAAATCGCCAGACAAACACGGTAGGCTCGGCTGTAA
CCGCTGTACTGGAGGGCAGCCGTCCTTTGCTGGTTGAGGTGCAGGCACTTACTAATACTACCAGTTTCGGCCAGCCGCGC
CGCACTGCCAACGGGGTGGATTTTAACCGCACCCTTATGATAGCTGCGGTCCTCTCCAAACGCCTGTCCATGCGTTTAGG
TACGCAGGACATTATTGTAAACGCCACCGGCGGTATCCGTTTGGATGAGCCGGCTGCAGATTTGGCTATTGCACTGGCTA
TTGCTTCCAGTTACCGGGATATCGGCGTATGCCCCGAAACTATCGCTCTGGGAGAGATTGGTCTTTCTGGGGAAATGCGG
ACTATTTCCCATCTGGAAAGGCGTCTTTCCGAGGCTAGCCGTTTGGGTTTTAAAAGGGCTTTGGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCAGTGAGGCTGGCACTTACCGGGGTGA
AAACGGAGACGGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-424)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C6EMF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.109

99.351

0.498
  radA Streptococcus pneumoniae Rx1

48.14

98.918

0.476
  radA Streptococcus pneumoniae D39

48.14

98.918

0.476
  radA Streptococcus pneumoniae R6

48.14

98.918

0.476
  radA Streptococcus pneumoniae TIGR4

48.14

98.918

0.476
  radA Streptococcus mitis SK321

48.14

98.918

0.476
  radA Streptococcus mitis NCTC 12261

47.921

98.918

0.474

Multiple sequence alignment