Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   E4T61_RS12185 Genome accession   NZ_CP039297
Coordinates   2515749..2516516 (-) Length   255 a.a.
NCBI ID   WP_012118014.1    Uniprot ID   A7Z6U2
Organism   Bacillus velezensis isolate UFLA258     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2510749..2521516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T61_RS12165 (E4T61_12165) - 2510951..2511763 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  E4T61_RS12170 (E4T61_12170) ccpN 2511789..2512427 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  E4T61_RS12175 (E4T61_12175) glyS 2512528..2514567 (-) 2040 WP_059368104.1 glycine--tRNA ligase subunit beta -
  E4T61_RS12180 (E4T61_12180) glyQ 2514560..2515447 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  E4T61_RS12185 (E4T61_12185) recO 2515749..2516516 (-) 768 WP_012118014.1 DNA repair protein RecO Machinery gene
  E4T61_RS12190 (E4T61_12190) - 2516553..2516696 (-) 144 WP_007408286.1 YqzL family protein -
  E4T61_RS12195 (E4T61_12195) era 2516852..2517757 (-) 906 WP_007408285.1 GTPase Era -
  E4T61_RS12200 (E4T61_12200) - 2517738..2518148 (-) 411 WP_059368102.1 cytidine deaminase -
  E4T61_RS12205 (E4T61_12205) - 2518272..2518646 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  E4T61_RS12210 (E4T61_12210) ybeY 2518627..2519100 (-) 474 WP_136396496.1 rRNA maturation RNase YbeY -
  E4T61_RS12215 (E4T61_12215) - 2519101..2521233 (-) 2133 WP_007408284.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29310.95 Da        Isoelectric Point: 8.5625

>NTDB_id=358024 E4T61_RS12185 WP_012118014.1 2515749..2516516(-) (recO) [Bacillus velezensis isolate UFLA258]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=358024 E4T61_RS12185 WP_012118014.1 2515749..2516516(-) (recO) [Bacillus velezensis isolate UFLA258]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACAGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAATGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z6U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886

Multiple sequence alignment