Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   E4T61_RS08890 Genome accession   NZ_CP039297
Coordinates   1814754..1815188 (+) Length   144 a.a.
NCBI ID   WP_136396382.1    Uniprot ID   -
Organism   Bacillus velezensis isolate UFLA258     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1809754..1820188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T61_RS08885 (E4T61_08885) - 1813254..1814465 (-) 1212 WP_136396381.1 cytochrome P450 -
  E4T61_RS08890 (E4T61_08890) nucA/comI 1814754..1815188 (+) 435 WP_136396382.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  E4T61_RS08895 (E4T61_08895) - 1815248..1816003 (+) 756 WP_136396383.1 YoaK family protein -
  E4T61_RS08900 (E4T61_08900) - 1816037..1816399 (-) 363 WP_136396384.1 hypothetical protein -
  E4T61_RS08905 (E4T61_08905) - 1816592..1817920 (-) 1329 WP_136396385.1 S8 family peptidase -
  E4T61_RS08910 (E4T61_08910) - 1818099..1818332 (+) 234 WP_021493648.1 hypothetical protein -
  E4T61_RS08915 (E4T61_08915) - 1818588..1819295 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  E4T61_RS08920 (E4T61_08920) - 1819355..1819807 (+) 453 WP_012117605.1 OsmC family protein -
  E4T61_RS08925 (E4T61_08925) - 1819821..1820174 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15544.47 Da        Isoelectric Point: 7.2418

>NTDB_id=358013 E4T61_RS08890 WP_136396382.1 1814754..1815188(+) (nucA/comI) [Bacillus velezensis isolate UFLA258]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=358013 E4T61_RS08890 WP_136396382.1 1814754..1815188(+) (nucA/comI) [Bacillus velezensis isolate UFLA258]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment