Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   E4A35_RS19880 Genome accession   NZ_CP039269
Coordinates   3840564..3840998 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus cereus strain MH19     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3835564..3845998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4A35_RS19860 - 3835963..3836520 (+) 558 WP_078437787.1 PadR family transcriptional regulator -
  E4A35_RS19865 - 3836734..3837165 (-) 432 WP_000438229.1 NUDIX hydrolase -
  E4A35_RS19870 metE 3837813..3840101 (+) 2289 WP_078437786.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  E4A35_RS19875 comJ 3840138..3840527 (-) 390 WP_000424033.1 competence protein ComJ -
  E4A35_RS19880 nucA/comI 3840564..3840998 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  E4A35_RS19885 - 3841235..3841984 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  E4A35_RS19890 kinB 3842038..3843312 (-) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  E4A35_RS19895 - 3843622..3843822 (+) 201 WP_000929281.1 hypothetical protein -
  E4A35_RS19900 - 3843945..3845108 (+) 1164 WP_070171702.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=357777 E4A35_RS19880 WP_000811492.1 3840564..3840998(-) (nucA/comI) [Bacillus cereus strain MH19]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=357777 E4A35_RS19880 WP_000811492.1 3840564..3840998(-) (nucA/comI) [Bacillus cereus strain MH19]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment