Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   E4A35_RS00020 Genome accession   NZ_CP039269
Coordinates   3012..4139 (+) Length   375 a.a.
NCBI ID   WP_000470750.1    Uniprot ID   A0A2C3GXQ0
Organism   Bacillus cereus strain MH19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4A35_RS00005 dnaA 1..1341 (+) 1341 WP_000428021.1 chromosomal replication initiator protein DnaA -
  E4A35_RS00010 dnaN 1520..2659 (+) 1140 WP_001212527.1 DNA polymerase III subunit beta -
  E4A35_RS00015 yaaA 2787..2999 (+) 213 WP_000821367.1 S4 domain-containing protein YaaA -
  E4A35_RS00020 recF 3012..4139 (+) 1128 WP_000470750.1 DNA replication/repair protein RecF Machinery gene
  E4A35_RS00025 gyrB 4178..6100 (+) 1923 WP_000435993.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  E4A35_RS00030 gyrA 6189..8660 (+) 2472 WP_001282851.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43334.66 Da        Isoelectric Point: 6.7809

>NTDB_id=357750 E4A35_RS00020 WP_000470750.1 3012..4139(+) (recF) [Bacillus cereus strain MH19]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=357750 E4A35_RS00020 WP_000470750.1 3012..4139(+) (recF) [Bacillus cereus strain MH19]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAAGTAAATGT
AATTATCGGCGAGAACGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTGGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGTGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGAAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATATTGCAAAAACGCTTTGAGTTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCAGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCGATTCTTTTACTCGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C3GXQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

66.304

98.133

0.651

Multiple sequence alignment