Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E6A54_RS01995 Genome accession   NZ_CP039261
Coordinates   413029..414408 (+) Length   459 a.a.
NCBI ID   WP_012845675.1    Uniprot ID   A0A1Z1N7N5
Organism   Lactobacillus johnsonii strain DC22.2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 408029..419408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6A54_RS01975 (E6A54_01960) pepC 408579..409928 (-) 1350 WP_158172141.1 aminopeptidase C -
  E6A54_RS01980 (E6A54_01965) - 410256..411305 (+) 1050 WP_087176735.1 IS30 family transposase -
  E6A54_RS09990 - 411302..411877 (+) 576 WP_267902525.1 LCP family protein -
  E6A54_RS09995 - 411907..412359 (+) 453 WP_267902526.1 LCP family protein -
  E6A54_RS01990 (E6A54_01975) - 412478..413029 (+) 552 WP_004895781.1 dUTP diphosphatase -
  E6A54_RS01995 (E6A54_01980) radA 413029..414408 (+) 1380 WP_012845675.1 DNA repair protein RadA Machinery gene
  E6A54_RS02000 (E6A54_01985) gltX 414490..415989 (+) 1500 WP_004895777.1 glutamate--tRNA ligase -
  E6A54_RS02005 (E6A54_01990) cysS 416079..417491 (+) 1413 WP_199247315.1 cysteine--tRNA ligase -
  E6A54_RS02010 (E6A54_01995) - 417484..417927 (+) 444 WP_004895772.1 Mini-ribonuclease 3 -
  E6A54_RS02015 (E6A54_02000) rlmB 417914..418669 (+) 756 WP_158172143.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50083.63 Da        Isoelectric Point: 7.2244

>NTDB_id=357698 E6A54_RS01995 WP_012845675.1 413029..414408(+) (radA) [Lactobacillus johnsonii strain DC22.2]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSAKATASRLITKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEYSVLYVSGEESASQIKMRADRLGVSDSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQDLKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=357698 E6A54_RS01995 WP_012845675.1 413029..414408(+) (radA) [Lactobacillus johnsonii strain DC22.2]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAGCTAAGGCAACTGCAAGCCGGTTGATAA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAATATAGCGTCTTATATGTTTCAGGAGAAGAGTCTGCGAGTC
AAATAAAGATGCGAGCAGATCGACTAGGAGTAAGCGATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAAAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGACTCTATTCAAACAATGAACGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAATTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGGCATGTAACTAAAGAAGGAGCAATTGCTGGTCCCAAGATTATGGAACATATGGTTGATACCGTCCTTTAC
TTTGAAGGGGATGGGCACCACTCATATCGAATTTTAAGATCAGTCAAGAATCGTTTTGGCGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGATCTTCAGTTGTCGTTTCACTTGAAGGAACGCGGCCGCTTCTTGCGGATATCCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGTATGGCGGTTGCTTCAAGTTACAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTA
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCTGCAAAAGTAGGCTTTAAGAGGATTTTTAT
CCCTAAAAACAACTTAAACCAGGACCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z1N7N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.699

99.782

0.606
  radA Streptococcus pneumoniae Rx1

60.699

99.782

0.606
  radA Streptococcus pneumoniae D39

60.699

99.782

0.606
  radA Streptococcus pneumoniae R6

60.699

99.782

0.606
  radA Streptococcus pneumoniae TIGR4

60.699

99.782

0.606
  radA Streptococcus mitis SK321

60.699

99.782

0.606
  radA Bacillus subtilis subsp. subtilis str. 168

56.579

99.346

0.562

Multiple sequence alignment