Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E5554_RS10325 Genome accession   NZ_CP039248
Coordinates   2127041..2128408 (-) Length   455 a.a.
NCBI ID   WP_010336315.1    Uniprot ID   A0A9X7U623
Organism   Sphingobium sp. PAMC28499     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2122041..2133408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5554_RS10305 (E5554_10305) - 2122251..2124191 (+) 1941 WP_136186712.1 tetratricopeptide repeat protein -
  E5554_RS10310 (E5554_10310) - 2124201..2125352 (+) 1152 WP_136186713.1 deoxyguanosinetriphosphate triphosphohydrolase -
  E5554_RS10315 (E5554_10315) - 2125943..2126329 (+) 387 WP_136186714.1 hypothetical protein -
  E5554_RS10320 (E5554_10320) - 2126506..2127030 (-) 525 WP_063977132.1 CvpA family protein -
  E5554_RS10325 (E5554_10325) radA 2127041..2128408 (-) 1368 WP_010336315.1 DNA repair protein RadA Machinery gene
  E5554_RS10330 (E5554_10330) - 2128498..2129199 (+) 702 WP_136186715.1 endonuclease/exonuclease/phosphatase family protein -
  E5554_RS10335 (E5554_10335) - 2129487..2129876 (+) 390 WP_136186716.1 hypothetical protein -
  E5554_RS10340 (E5554_10340) - 2130113..2131354 (-) 1242 WP_136186717.1 hypothetical protein -
  E5554_RS10345 (E5554_10345) - 2131496..2131675 (-) 180 WP_348769748.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47779.70 Da        Isoelectric Point: 6.7474

>NTDB_id=357592 E5554_RS10325 WP_010336315.1 2127041..2128408(-) (radA) [Sphingobium sp. PAMC28499]
MAKAKRKFVCQQCGTVSGRWQGQCDDCGEWNSIVEEAAETVFSAKHDLQGGGRAITLIGLDSKVELPSRTSTGIAEFDRA
LGGGIVPGSATLIGGDPGIGKSTLLLQAAARVASRGLTVAYISGEEASDQVRLRAQRLGLGNAPVQLASATSVRDILTTL
SEGVPPALLIIDSIQTMHSDLIEGAPGTVSQVRASSQELIKFAKQRGTALILVGHVTKDGSIAGPRVLEHMVDTVLAFEG
ERSHQYRILRAIKNRFGGTDEIGVFSMVAEGLEEVANPSALFLTNRDETVTGATVFPALEGTRPVLVEIQALVVRLSSGA
TPRRAVVGWDSGRLAMILAVLEARCGLSFSTCEVYLNVAGGYRLSDPAADLAVAAALMSALSERPVPADVVLFGEIALSS
EIRPVSHTPLRLRESAKLGFNRAFVPASGADGVKGISVSGFRTLAQLVDQMLGRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=357592 E5554_RS10325 WP_010336315.1 2127041..2128408(-) (radA) [Sphingobium sp. PAMC28499]
ATGGCCAAGGCAAAGCGCAAATTCGTCTGTCAACAATGCGGCACCGTTTCCGGCCGGTGGCAGGGGCAGTGCGATGACTG
TGGCGAATGGAACAGCATCGTCGAGGAAGCGGCCGAGACCGTCTTTTCCGCCAAGCATGACCTGCAGGGCGGCGGCCGGG
CGATCACGCTCATCGGCCTCGACAGCAAGGTGGAACTGCCCTCGCGCACCAGCACCGGCATTGCCGAGTTCGATCGGGCG
CTGGGCGGCGGCATCGTGCCGGGATCGGCCACGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACCCTGTTGTTGCA
GGCGGCAGCGCGGGTCGCCTCGCGCGGGCTGACCGTCGCCTATATCAGCGGCGAGGAGGCGTCGGACCAGGTGCGGCTGC
GTGCCCAGCGGCTGGGGCTGGGCAATGCGCCGGTGCAACTGGCGAGCGCGACATCGGTGCGCGATATCCTGACGACGCTG
AGCGAGGGCGTGCCGCCCGCCTTGCTGATCATCGATTCGATCCAGACCATGCACAGCGACCTGATCGAGGGCGCGCCAGG
CACCGTGAGCCAGGTGCGCGCGTCGAGCCAGGAACTGATCAAATTCGCCAAGCAGCGCGGCACCGCGCTGATCCTGGTCG
GCCATGTCACCAAGGATGGCAGCATCGCCGGCCCGCGCGTGCTGGAACATATGGTCGATACGGTGCTGGCGTTCGAGGGT
GAGCGCAGCCACCAATATCGCATCCTGCGCGCGATCAAGAACCGCTTTGGCGGCACCGACGAGATCGGCGTCTTCTCGAT
GGTTGCCGAGGGGCTGGAGGAGGTCGCCAATCCGTCCGCTTTGTTCCTCACCAATCGCGACGAGACGGTGACGGGCGCCA
CCGTCTTCCCCGCGCTCGAAGGAACGCGGCCGGTGCTGGTCGAGATCCAGGCGCTGGTGGTGCGGCTGTCGAGCGGGGCG
ACGCCCCGGCGCGCGGTGGTCGGCTGGGACAGCGGCCGGCTGGCGATGATCCTGGCCGTGCTGGAGGCGCGCTGCGGGCT
CAGCTTCTCGACCTGCGAAGTCTATCTCAACGTCGCGGGCGGCTATCGGCTGTCCGATCCGGCCGCCGACCTGGCCGTCG
CCGCCGCTTTGATGTCGGCCTTGTCGGAACGGCCGGTGCCCGCCGATGTCGTGCTGTTCGGCGAGATTGCGCTGTCGAGC
GAAATTCGCCCCGTCTCGCACACGCCGCTGCGGCTGCGGGAATCGGCGAAACTGGGCTTCAACCGCGCCTTCGTGCCGGC
GTCGGGCGCGGACGGGGTGAAGGGGATTTCGGTCAGCGGCTTTCGCACCCTTGCACAGCTGGTTGACCAGATGCTCGGGC
GCGGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(82-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.403

100

0.481
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464
  radA Streptococcus pneumoniae Rx1

46.053

100

0.462
  radA Streptococcus pneumoniae D39

46.053

100

0.462
  radA Streptococcus pneumoniae R6

46.053

100

0.462
  radA Streptococcus pneumoniae TIGR4

46.053

100

0.462
  radA Streptococcus mitis SK321

45.833

100

0.459

Multiple sequence alignment