Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   E5491_RS00250 Genome accession   NZ_CP039156
Coordinates   53324..54673 (-) Length   449 a.a.
NCBI ID   WP_000806247.1    Uniprot ID   -
Organism   Staphylococcus aureus strain WCUH29     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 24476..82057 53324..54673 within 0
SCCmec 44969..61457 53324..54673 within 0


Gene organization within MGE regions


Location: 24476..82057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5491_RS00110 (E5491_00110) yycF 24934..25635 (+) 702 WP_000101976.1 response regulator YycF -
  E5491_RS00115 (E5491_00115) walK 25648..27474 (+) 1827 WP_000871607.1 cell wall metabolism sensor histidine kinase WalK -
  E5491_RS00120 (E5491_00120) yycH 27467..28801 (+) 1335 WP_001060140.1 two-component system activity regulator YycH -
  E5491_RS00125 (E5491_00125) - 28802..29590 (+) 789 WP_001104165.1 two-component system regulatory protein YycI -
  E5491_RS00130 (E5491_00130) - 29979..30779 (+) 801 WP_000088649.1 MBL fold metallo-hydrolase -
  E5491_RS00135 (E5491_00135) adsA 31006..33324 (+) 2319 WP_000645787.1 LPXTG-anchored adenosine synthase AdsA -
  E5491_RS00140 (E5491_00140) rlmH 33692..34171 (+) 480 WP_000704775.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  E5491_RS00145 (E5491_00145) - 34391..35647 (+) 1257 WP_000566670.1 DUF3578 domain-containing protein -
  E5491_RS00150 (E5491_00150) - 36062..36301 (+) 240 WP_001282728.1 hypothetical protein -
  E5491_RS00155 (E5491_00155) - 36333..37007 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  E5491_RS00160 (E5491_00160) - 37238..38221 (+) 984 WP_000336291.1 protein rep -
  E5491_RS00165 (E5491_00165) aadD1 38381..39151 (+) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  E5491_RS00170 (E5491_00170) bleO 39368..39772 (+) 405 WP_001242578.1 bleomycin binding protein -
  E5491_RS00175 (E5491_00175) mobV 40279..41541 (+) 1263 WP_000119405.1 MobV family relaxase -
  E5491_RS00180 (E5491_00180) - 41679..42353 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  E5491_RS00185 (E5491_00185) - 42437..42778 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  E5491_RS00195 (E5491_00195) - 43655..44398 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  E5491_RS00200 (E5491_00200) - 44495..44923 (+) 429 WP_000872606.1 MaoC family dehydratase -
  E5491_RS00205 (E5491_00205) mecA 44969..46978 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  E5491_RS00210 (E5491_00210) - 47075..48061 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  E5491_RS00215 (E5491_00215) - 48033..48293 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  E5491_RS00220 (E5491_00220) - 48284..49891 (-) 1608 Protein_38 IS1182 family transposase -
  E5491_RS00225 (E5491_00225) - 49943..50449 (-) 507 WP_001092195.1 DUF1643 domain-containing protein -
  E5491_RS00230 (E5491_00230) - 50464..50775 (-) 312 WP_001024803.1 DUF960 family protein -
  E5491_RS00240 (E5491_00240) - 50862..51212 (-) 351 WP_000859157.1 SAUGI family uracil-DNA glycosylase inhibitor -
  E5491_RS00245 (E5491_00245) - 51678..53302 (-) 1625 Protein_42 recombinase family protein -
  E5491_RS00250 (E5491_00250) ccrA 53324..54673 (-) 1350 WP_000806247.1 cassette chromosome recombinase CcrA Machinery gene
  E5491_RS00255 (E5491_00255) - 54861..56633 (-) 1773 WP_000195098.1 cassette chromosome replicative helicase -
  E5491_RS00260 (E5491_00260) - 56633..56929 (-) 297 WP_000221967.1 cassette chromosome ssDNA-binding protein -
  E5491_RS00265 (E5491_00265) - 57101..58684 (+) 1584 WP_000271877.1 hypothetical protein -
  E5491_RS00270 (E5491_00270) - 58756..59688 (+) 933 WP_000364405.1 hypothetical protein -
  E5491_RS00275 (E5491_00275) - 60114..60491 (+) 378 WP_000687965.1 hypothetical protein -
  E5491_RS00280 (E5491_00280) - 60960..61457 (+) 498 WP_000051479.1 hypothetical protein -
  E5491_RS00285 (E5491_00285) - 61817..61951 (-) 135 WP_001790628.1 hypothetical protein -
  E5491_RS00290 (E5491_00290) - 61915..62007 (+) 93 WP_001790000.1 hypothetical protein -
  E5491_RS00295 (E5491_00295) - 62154..63899 (+) 1746 WP_000700190.1 DUF6119 family protein -
  E5491_RS00300 (E5491_00300) - 63997..64352 (-) 356 Protein_53 tRNA-dihydrouridine synthase -
  E5491_RS00305 (E5491_00305) - 64657..64745 (-) 89 Protein_54 hypothetical protein -
  E5491_RS00310 (E5491_00310) - 64843..65598 (-) 756 WP_000355845.1 sulfite exporter TauE/SafE family protein -
  E5491_RS00315 (E5491_00315) cstR 65598..65858 (-) 261 WP_001105088.1 persulfide-sensing transcriptional repressor CstR -
  E5491_RS00320 (E5491_00320) cstA 65995..67059 (+) 1065 WP_000816133.1 persulfide response sulfurtransferase CstA -
  E5491_RS00325 (E5491_00325) cstB 67090..68424 (+) 1335 WP_000465472.1 persulfide dioxygenase-sulfurtransferase CstB -
  E5491_RS00330 (E5491_00330) sqr 68443..69636 (+) 1194 WP_001033018.1 type II sulfide:quinone oxidoreductase Sqr -
  E5491_RS15460 - 69903..70117 (-) 215 Protein_60 universal stress protein -
  E5491_RS00345 (E5491_00345) - 70303..71289 (-) 987 WP_000662022.1 tRNA-dihydrouridine synthase -
  E5491_RS00350 (E5491_00350) - 71598..71801 (+) 204 WP_000389759.1 DUF6007 family protein -
  E5491_RS00355 (E5491_00355) - 71850..72146 (+) 297 WP_000201644.1 TfoX/Sxy family protein -
  E5491_RS00360 (E5491_00360) - 72354..72956 (+) 603 WP_000908956.1 hypothetical protein -
  E5491_RS00365 (E5491_00365) - 73223..76375 (+) 3153 WP_000080564.1 ATP-binding protein -
  E5491_RS00370 (E5491_00370) - 76432..76914 (+) 483 WP_000410567.1 hypothetical protein -
  E5491_RS00375 (E5491_00375) - 77120..78106 (+) 987 WP_000710555.1 phosphatidylinositol-specific phospholipase C -
  E5491_RS00380 (E5491_00380) - 78327..79097 (+) 771 WP_000977777.1 tandem-type lipoprotein -
  E5491_RS00385 (E5491_00385) - 79149..79919 (+) 771 WP_000597207.1 tandem-type lipoprotein -
  E5491_RS00390 (E5491_00390) - 79984..80754 (+) 771 WP_031790901.1 tandem-type lipoprotein -
  E5491_RS00395 (E5491_00395) - 80819..81589 (+) 771 WP_000977803.1 tandem-type lipoprotein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52580.41 Da        Isoelectric Point: 9.9370

>NTDB_id=357236 E5491_RS00250 WP_000806247.1 53324..54673(-) (ccrA) [Staphylococcus aureus strain WCUH29]
MKQAIGYLRQSTTKQQSLAAQKQTIEALAKKHNIQYITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKMITTHAPFGYHYH
NGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKFINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTI
YEEAQVTRTQKPVKRKPSENQLKQKIKCPYCDSTLTNMTIRKKHHTLRYYVCPQNMNASRFVCEFKGINAQELETSVLAT
CQDFFQNQQLYSKINHTIQQRLKRQRDIETKTTLNHEQLIEKLAQDKIDAETFREQTQSLRQQSKPISSISTYQIRKAFQ
NIIQQRFTLNMLYPYIDEINISKNKSLAGIYFKNEPLNIVKQTMELSIV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=357236 E5491_RS00250 WP_000806247.1 53324..54673(-) (ccrA) [Staphylococcus aureus strain WCUH29]
ATGAAACAAGCAATAGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCACAAAAACAAACCATCGAGGC
ATTAGCCAAAAAACATAATATTCAATACATTACCTTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGGAACGGTT
ACCAACAAATTACTGAACTGATTCAACAAGGACAATGTGATGTATTATGTTGTTACAGATTAAACCGACTTCATCGCAAT
CTTAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAATACCATGTCCATATCTTAAGCGTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCCGAACTAGAATCTGATAATATAGGCGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAAATGATTACAACACATGCACCCTTTGGGTATCACTATCAT
AATGGTACTTTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTATTACCTTCAAGGTTATGGTTA
TAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAATTCATTAATCGTAAGCCCTATCAAGTGCGTAATATTATCCTTA
ACCCTAATTACTGTGGCCGTGTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACCAGTAAAACGTAAGCCGTCAGAAAACCAACTCAAACAAAAAATCAA
ATGTCCTTATTGTGATTCAACACTAACTAATATGACCATTAGAAAAAAGCACCATACATTACGTTATTATGTTTGTCCTC
AAAATATGAATGCATCTCGTTTTGTCTGTGAATTCAAAGGGATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCAACAACGACTCAAAAGACAAAGAGA
TATAGAAACTAAAACTACACTCAATCACGAGCAGCTGATAGAAAAATTAGCCCAAGACAAAATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAGCAATCAAAACCTATATCATCAATCAGTACGTATCAAATTCGAAAAGCTTTCCAA
AACATCATTCAACAACGTTTCACGCTAAACATGTTGTACCCCTATATTGATGAAATTAATATTTCTAAAAATAAAAGCCT
CGCTGGCATCTATTTCAAAAATGAACCACTGAATATTGTAAAGCAGACTATGGAATTATCAATTGTATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(170-249)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

100

100

1
  ccrA Staphylococcus aureus N315

74.833

100

0.748

Multiple sequence alignment