Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E5Y90_RS02650 Genome accession   NZ_CP039143
Coordinates   571091..571864 (+) Length   257 a.a.
NCBI ID   WP_373688388.1    Uniprot ID   -
Organism   Acinetobacter sp. 10FS3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 566091..576864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Y90_RS02620 (E5Y90_02660) - 566350..566763 (+) 414 WP_174659326.1 hypothetical protein -
  E5Y90_RS02625 (E5Y90_02665) - 567009..568289 (+) 1281 WP_174659327.1 glycosyltransferase family 4 protein -
  E5Y90_RS02630 (E5Y90_02670) - 568308..568859 (+) 552 WP_151204105.1 phosphatase PAP2 family protein -
  E5Y90_RS02635 (E5Y90_02675) iscX 568949..569149 (+) 201 WP_151204106.1 Fe-S cluster assembly protein IscX -
  E5Y90_RS02640 (E5Y90_02680) ndk 569260..569691 (+) 432 WP_035265623.1 nucleoside-diphosphate kinase -
  E5Y90_RS02645 (E5Y90_02685) rlmN 569814..571049 (+) 1236 WP_174659328.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E5Y90_RS02650 (E5Y90_02690) pilF 571091..571864 (+) 774 WP_373688388.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E5Y90_RS02655 (E5Y90_02695) - 571855..572646 (+) 792 WP_174659329.1 helix-turn-helix domain-containing protein -
  E5Y90_RS02660 (E5Y90_02700) ispG 572668..573789 (+) 1122 WP_151204110.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E5Y90_RS02665 (E5Y90_02705) hisS 573793..575088 (+) 1296 WP_174659330.1 histidine--tRNA ligase -
  E5Y90_RS02670 (E5Y90_02710) - 575119..575817 (+) 699 WP_174659331.1 YfgM family protein -

Sequence

Protein


Download         Length: 257 a.a.        Molecular weight: 29170.07 Da        Isoelectric Point: 9.0690

>NTDB_id=357203 E5Y90_RS02650 WP_373688388.1 571091..571864(+) (pilF) [Acinetobacter sp. 10FS3-1]
MISVLCGFWGLTACQTPELGQKDPEKATKVRTQLAAEYLKSGDLDSAKRALDQALESNSRDSQANMMMGVLLQQEGSPLN
LEKAETYFKRAISADPKNAQARNNYGTYLYQLGRYNDAIEQFKIAGATLGYDQRFRALENMGRIYLKTGDVVNAEKTFKQ
ALQANRDSYISMLELAEIFYLNQQFPAATQMYEHFVRGVGQKNQSARALWIGIRTARANGDRLGMQVLVNQLRALFPESQ
EYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 774 bp        

>NTDB_id=357203 E5Y90_RS02650 WP_373688388.1 571091..571864(+) (pilF) [Acinetobacter sp. 10FS3-1]
TTGATTTCAGTGCTGTGTGGGTTTTGGGGGTTAACCGCGTGCCAAACGCCAGAACTGGGTCAGAAAGACCCGGAAAAAGC
CACTAAAGTCCGTACACAATTGGCGGCAGAATATCTTAAATCAGGTGATCTGGATTCAGCAAAACGTGCGCTCGATCAGG
CACTGGAAAGCAATTCGCGTGATTCTCAGGCCAACATGATGATGGGTGTGCTGCTTCAGCAGGAAGGCAGTCCACTCAAT
CTGGAAAAAGCCGAGACTTATTTCAAGCGTGCCATTTCCGCAGACCCGAAAAATGCACAGGCGCGAAATAATTACGGTAC
CTATTTATATCAGCTTGGGCGCTATAATGATGCGATTGAGCAGTTTAAAATTGCGGGTGCAACTTTAGGGTATGATCAGC
GATTTCGTGCCCTAGAGAATATGGGCCGTATTTATCTGAAAACTGGCGATGTTGTGAATGCTGAAAAAACATTCAAACAG
GCGCTACAGGCTAATCGCGATTCGTATATATCAATGTTAGAGTTGGCAGAAATTTTTTATCTGAACCAGCAGTTCCCGGC
AGCGACACAAATGTATGAACATTTTGTGCGTGGGGTGGGACAGAAAAATCAGAGTGCCCGCGCACTCTGGATTGGCATTC
GTACCGCGCGTGCCAATGGTGATCGGCTAGGAATGCAGGTATTGGTTAATCAATTGCGTGCACTCTTTCCTGAAAGCCAG
GAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(34-71)

Tetratricopeptide repeat (TPR_16)

(80-124)

Tetratricopeptide repeat (TPR_16)

(141-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

81.301

95.72

0.778
  pilF Acinetobacter baylyi ADP1

72.549

99.222

0.72

Multiple sequence alignment