Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   GQF94_RS15395 Genome accession   NZ_CP047002
Coordinates   3194724..3196553 (+) Length   609 a.a.
NCBI ID   WP_001617666.1    Uniprot ID   -
Organism   Escherichia coli strain J-8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3189724..3201553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQF94_RS15365 (GQF94_15345) - 3189998..3190846 (-) 849 WP_001059699.1 YPO3983 family protein -
  GQF94_RS15370 (GQF94_15350) - 3191027..3191179 (+) 153 Protein_3032 CorA family divalent cation transporter -
  GQF94_RS15375 (GQF94_15355) - 3191805..3192104 (+) 300 WP_000526434.1 hypothetical protein -
  GQF94_RS15380 (GQF94_15360) rarD 3192151..3193038 (-) 888 WP_000339103.1 EamA family transporter RarD -
  GQF94_RS15385 (GQF94_15365) yigI 3193090..3193557 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  GQF94_RS15390 (GQF94_15370) pldA 3193722..3194591 (+) 870 WP_001259700.1 phospholipase A -
  GQF94_RS15395 (GQF94_15375) recQ 3194724..3196553 (+) 1830 WP_001617666.1 ATP-dependent DNA helicase RecQ Machinery gene
  GQF94_RS15400 (GQF94_15380) rhtC 3196617..3197237 (+) 621 WP_000928824.1 threonine export protein RhtC -
  GQF94_RS15405 (GQF94_15385) rhtB 3197299..3197919 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  GQF94_RS15410 (GQF94_15390) pldB 3198030..3199052 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  GQF94_RS15415 (GQF94_15395) yigL 3199060..3199860 (+) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  GQF94_RS15420 (GQF94_15400) bioP 3199936..3200835 (+) 900 WP_001196238.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68376.51 Da        Isoelectric Point: 7.2543

>NTDB_id=356800 GQF94_RS15395 WP_001617666.1 3194724..3196553(+) (recQ) [Escherichia coli strain J-8]
MAQADVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=356800 GQF94_RS15395 WP_001617666.1 3194724..3196553(+) (recQ) [Escherichia coli strain J-8]
GTGGCGCAGGCGGATGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAACAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCTT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTACTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGTTTTGGTATGGGCTATGTGGTGGAAGTGATTCGCGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTAAAAGTCTACGGTATGGGCCGTGATAAAAGTCATGAACATTGGGTGAGCGTGATCCGCCAGTTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTCCAACTGACAGAGGCCGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391