Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FI9785_RS01705 Genome accession   NC_013504
Coordinates   359275..360654 (+) Length   459 a.a.
NCBI ID   WP_012845675.1    Uniprot ID   A0A1Z1N7N5
Organism   Lactobacillus johnsonii FI9785     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 354275..365654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FI9785_RS01685 (FI9785_412) - 355505..356065 (-) 561 WP_012845671.1 low temperature requirement protein A -
  FI9785_RS01690 (FI9785_413) pepC 356225..357574 (-) 1350 WP_012845672.1 aminopeptidase C -
  FI9785_RS01695 (FI9785_414) - 357772..358605 (+) 834 WP_012845673.1 LCP family protein -
  FI9785_RS01700 (FI9785_415) - 358724..359275 (+) 552 WP_012845674.1 dUTP diphosphatase -
  FI9785_RS01705 (FI9785_416) radA 359275..360654 (+) 1380 WP_012845675.1 DNA repair protein RadA Machinery gene
  FI9785_RS01710 (FI9785_417) gltX 360736..362235 (+) 1500 WP_004895777.1 glutamate--tRNA ligase -
  FI9785_RS01715 (FI9785_418) cysS 362327..363739 (+) 1413 WP_012845676.1 cysteine--tRNA ligase -
  FI9785_RS01720 (FI9785_419) - 363732..364175 (+) 444 WP_012845677.1 ribonuclease III domain-containing protein -
  FI9785_RS01725 (FI9785_420) rlmB 364162..364917 (+) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50083.63 Da        Isoelectric Point: 7.2244

>NTDB_id=35678 FI9785_RS01705 WP_012845675.1 359275..360654(+) (radA) [Lactobacillus johnsonii FI9785]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSAKATASRLITKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEYSVLYVSGEESASQIKMRADRLGVSDSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQDLKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=35678 FI9785_RS01705 WP_012845675.1 359275..360654(+) (radA) [Lactobacillus johnsonii FI9785]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAGCTAAGGCAACTGCAAGCCGGTTGATAA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAATATAGCGTCTTATATGTTTCAGGAGAAGAGTCTGCGAGTC
AAATAAAGATGCGAGCAGATCGACTAGGAGTAAGCGATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGACTCTATTCAAACAATGAACGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAATTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGGCATGTAACTAAAGAAGGAGCAATTGCTGGTCCCAAGATTATGGAACATATGGTTGATACCGTCCTTTAC
TTTGAAGGGGATGGGCACCACTCATATCGAATTTTAAGATCAGTCAAGAATCGTTTTGGCGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGATCTTCAGTTGTCGTTTCACTTGAAGGAACGCGGCCGCTTCTTGCGGATATTCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGTATGGCGGTTGCTTCAAGTTACAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTA
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCTGCAAAAGTAGGCTTTAAGAGGATTTTTAT
CCCTAAAAACAACTTAAACCAGGACCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z1N7N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.699

99.782

0.606
  radA Streptococcus pneumoniae Rx1

60.699

99.782

0.606
  radA Streptococcus pneumoniae D39

60.699

99.782

0.606
  radA Streptococcus pneumoniae R6

60.699

99.782

0.606
  radA Streptococcus pneumoniae TIGR4

60.699

99.782

0.606
  radA Streptococcus mitis SK321

60.699

99.782

0.606
  radA Bacillus subtilis subsp. subtilis str. 168

56.579

99.346

0.562

Multiple sequence alignment