Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GPY41_RS19335 Genome accession   NZ_CP046835
Coordinates   2423499..2425223 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain 07-2444     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418499..2430223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY41_RS19300 - 2418851..2419540 (-) 690 WP_158123865.1 PilW family protein -
  GPY41_RS19305 - 2419540..2420106 (-) 567 WP_103198169.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY41_RS19310 - 2420106..2420591 (-) 486 WP_158123866.1 type II secretion system protein -
  GPY41_RS19315 - 2420643..2421113 (-) 471 WP_011151246.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY41_RS19320 - 2421147..2421725 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY41_RS19325 - 2421804..2422265 (-) 462 WP_158123867.1 MSHA biogenesis protein MshF -
  GPY41_RS19330 - 2422269..2423489 (-) 1221 WP_158123868.1 type II secretion system F family protein -
  GPY41_RS19335 pilF 2423499..2425223 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  GPY41_RS19340 - 2425220..2426341 (-) 1122 WP_158123869.1 lipopolysaccharide assembly protein LapB -
  GPY41_RS19345 - 2426338..2427186 (-) 849 WP_158123870.1 ExeA family protein -
  GPY41_RS19350 mshL 2427194..2428840 (-) 1647 WP_026050229.1 pilus (MSHA type) biogenesis protein MshL -
  GPY41_RS19355 - 2428856..2429176 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  GPY41_RS19360 gspM 2429169..2429819 (-) 651 WP_045622003.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=356256 GPY41_RS19335 WP_011079397.1 2423499..2425223(-) (pilF) [Vibrio vulnificus strain 07-2444]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=356256 GPY41_RS19335 WP_011079397.1 2423499..2425223(-) (pilF) [Vibrio vulnificus strain 07-2444]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
TTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTCATCGCGCCAGAAA
GGCAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAG
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGGGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGTCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAATTTGGATATTTCAGAAAAACGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAATCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCAGACGATCTTCTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACAGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAGTTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAGCATGGAATGATGGACGCGTTGCGTGCCAATGATGCTGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371