Detailed information NTG0035623

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: LL3_RS01375
• Genome accession: NC_017190
• Coordinates: 258743..259864 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens LL3
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 253743..264864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL3_RS01355 (LL3_00260)	-	254063..255058 (-)	996	WP_013350915.1	DUF5105 domain-containing protein	-
LL3_RS01360 (LL3_00261)	-	255176..256573 (+)	1398	WP_014469698.1	YcdB/YcdC domain-containing protein	-
LL3_RS01365 (LL3_00262)	-	256775..258133 (+)	1359	WP_014469699.1	YcdB/YcdC domain-containing protein	-
LL3_RS01370 (LL3_00263)	-	258130..258633 (-)	504	WP_014469700.1	M15 family metallopeptidase	-
LL3_RS01375 (LL3_00264)	rapF	258743..259864 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
LL3_RS01380 (LL3_00265)	-	259978..260757 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
LL3_RS01385 (LL3_00266)	-	260841..262520 (+)	1680	WP_013350921.1	alpha-glucosidase	-
LL3_RS01390 (LL3_00267)	-	262505..262999 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
LL3_RS01395 (LL3_00268)	-	263000..263545 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
LL3_RS01400 (LL3_00269)	-	263754..264713 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=35623 LL3_RS01375 WP_013350919.1 258743..259864(+) (rapF) [Bacillus amyloliquefaciens LL3]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=35623 LL3_RS01375 WP_013350919.1 258743..259864(+) (rapF) [Bacillus amyloliquefaciens LL3]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402