Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GPY16_RS02435 Genome accession   NZ_CP046832
Coordinates   506464..508188 (+) Length   574 a.a.
NCBI ID   WP_158104952.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 06-2410     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501464..513188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY16_RS02410 gspM 501869..502518 (+) 650 Protein_433 type II secretion system protein GspM -
  GPY16_RS02415 - 502511..502831 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  GPY16_RS02420 mshL 502847..504493 (+) 1647 WP_026050229.1 pilus (MSHA type) biogenesis protein MshL -
  GPY16_RS02425 - 504501..505349 (+) 849 WP_038969544.1 ExeA family protein -
  GPY16_RS02430 - 505346..506467 (+) 1122 WP_158104951.1 tetratricopeptide repeat protein -
  GPY16_RS02435 pilF 506464..508188 (+) 1725 WP_158104952.1 GspE/PulE family protein Machinery gene
  GPY16_RS02440 - 508198..509418 (+) 1221 WP_038940912.1 type II secretion system F family protein -
  GPY16_RS02445 - 509422..509883 (+) 462 WP_094867743.1 MSHA biogenesis protein MshF -
  GPY16_RS02450 - 509962..510540 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY16_RS02455 - 510574..511044 (+) 471 WP_094867741.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY16_RS02460 - 511096..511581 (+) 486 WP_039549915.1 type II secretion system protein -
  GPY16_RS02465 - 511581..512147 (+) 567 WP_158104953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY16_RS02470 - 512147..512836 (+) 690 WP_039469689.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63744.10 Da        Isoelectric Point: 5.2320

>NTDB_id=356139 GPY16_RS02435 WP_158104952.1 506464..508188(+) (pilF) [Vibrio vulnificus strain 06-2410]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHEMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=356139 GPY16_RS02435 WP_158104952.1 506464..508188(+) (pilF) [Vibrio vulnificus strain 06-2410]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGATCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCACAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCTACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATAGGCCGTAACGGCGACACGCTGCGCATTGCAATGAGTGA
TCCTGCCGACCTGTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGGCAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGCTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAG
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGTGATGAGGTAACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGATGCCAATGTGTTGCGCTTGCGTCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCGCAAGATGGCCGTTTTAACATCCGATCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACAGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAGTTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGCTTCCCGAATCAAGTG
GAAGCCGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAGCATGAAATGATGGACGCGTTGCGTGCCAATGATGCTGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.283

97.387

0.373
  pilB Vibrio cholerae strain A1552

37.876

98.432

0.373