Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   E5L67_RS07635 Genome accession   NZ_CP038868
Coordinates   1464773..1465717 (-) Length   314 a.a.
NCBI ID   WP_162284669.1    Uniprot ID   -
Organism   Campylobacter coli strain 16SHKX65C     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1454638..1500590 1464773..1465717 within 0


Gene organization within MGE regions


Location: 1454638..1500590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5L67_RS07585 - 1454638..1456194 (-) 1557 WP_002831605.1 DNA polymerase III subunit gamma/tau -
  E5L67_RS07590 rho 1456197..1457495 (-) 1299 WP_002780613.1 transcription termination factor Rho -
  E5L67_RS07595 - 1457608..1459971 (+) 2364 WP_131260483.1 heavy metal translocating P-type ATPase -
  E5L67_RS07600 - 1459964..1460164 (+) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -
  E5L67_RS07605 - 1460194..1460493 (-) 300 WP_002777238.1 cytochrome c -
  E5L67_RS07610 - 1460570..1461133 (+) 564 WP_002820231.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  E5L67_RS07615 rfaD 1461130..1462083 (+) 954 WP_002831601.1 ADP-glyceromanno-heptose 6-epimerase -
  E5L67_RS07620 rfaE1 1462076..1463461 (+) 1386 WP_002831599.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  E5L67_RS07625 gmhA 1463458..1464018 (+) 561 WP_052778106.1 D-sedoheptulose 7-phosphate isomerase -
  E5L67_RS07630 - 1464018..1464780 (+) 763 Protein_1480 glycosyltransferase family 25 protein -
  E5L67_RS07635 waaF 1464773..1465717 (-) 945 WP_162284669.1 lipopolysaccharide heptosyltransferase II Regulator
  E5L67_RS07640 - 1465777..1466598 (+) 822 WP_002844798.1 glycosyltransferase family 2 protein -
  E5L67_RS07645 - 1466641..1468008 (+) 1368 WP_002844799.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  E5L67_RS07650 gmd 1468009..1469148 (+) 1140 WP_002844800.1 GDP-mannose 4,6-dehydratase -
  E5L67_RS07655 - 1469141..1470079 (+) 939 WP_002844801.1 NAD-dependent epimerase/dehydratase family protein -
  E5L67_RS07660 - 1470072..1471241 (+) 1170 WP_002844803.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  E5L67_RS07665 - 1471619..1472638 (+) 1020 WP_002844805.1 glycosyltransferase family 10 domain-containing protein -
  E5L67_RS07670 - 1472631..1473623 (+) 993 WP_223206634.1 alpha-1,2-fucosyltransferase -
  E5L67_RS07675 rfbF 1473620..1474435 (+) 816 WP_002844809.1 glucose-1-phosphate cytidylyltransferase -
  E5L67_RS07680 rfbG 1474439..1475512 (+) 1074 WP_162284670.1 CDP-glucose 4,6-dehydratase -
  E5L67_RS07685 - 1475529..1477286 (+) 1758 WP_002844812.1 thiamine pyrophosphate-binding protein -
  E5L67_RS07690 - 1477290..1478207 (+) 918 WP_002844814.1 NAD-dependent epimerase/dehydratase family protein -
  E5L67_RS07695 rfbH 1478210..1479532 (+) 1323 WP_002844816.1 lipopolysaccharide biosynthesis protein RfbH -
  E5L67_RS07700 - 1479548..1480621 (+) 1074 WP_002844818.1 glycosyltransferase family 2 protein -
  E5L67_RS07705 - 1480669..1481880 (+) 1212 WP_002844820.1 glycosyltransferase family 8 protein -
  E5L67_RS07710 - 1481867..1482919 (-) 1053 WP_004291473.1 glycosyltransferase family 4 protein -
  E5L67_RS07715 - 1482916..1484463 (-) 1548 WP_002844824.1 glycosyltransferase -
  E5L67_RS07720 - 1484460..1485347 (-) 888 WP_002777219.1 lipid A biosynthesis lauroyl acyltransferase -
  E5L67_RS07725 waaC 1485340..1486368 (-) 1029 WP_002787741.1 lipopolysaccharide heptosyltransferase I -
  E5L67_RS07730 - 1486433..1487224 (+) 792 WP_032684756.1 3'-5' exonuclease -
  E5L67_RS07735 galE 1487281..1488267 (+) 987 WP_002777216.1 UDP-glucose 4-epimerase GalE -
  E5L67_RS07740 - 1488261..1489961 (+) 1701 WP_002786296.1 ABC transporter ATP-binding protein -
  E5L67_RS07745 - 1489958..1491034 (+) 1077 WP_002787737.1 glycosyltransferase -
  E5L67_RS07750 - 1491027..1491956 (+) 930 WP_002777213.1 glycosyltransferase family 2 protein -
  E5L67_RS07755 - 1491944..1493041 (+) 1098 WP_002786294.1 glycosyltransferase -
  E5L67_RS07760 - 1493045..1495189 (+) 2145 WP_002787735.1 STT3 domain-containing protein -
  E5L67_RS07765 pglA 1495201..1496331 (+) 1131 WP_002777209.1 N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase -
  E5L67_RS07770 pglC 1496324..1496926 (+) 603 WP_002777206.1 undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase -
  E5L67_RS07775 pglD 1496913..1497500 (+) 588 WP_002780649.1 UDP-N-acetylbacillosamine N-acetyltransferase -
  E5L67_RS07780 pglE 1497655..1498815 (+) 1161 WP_002777202.1 UDP-N-acetylbacillosamine transaminase -
  E5L67_RS07785 pglF 1498818..1500590 (+) 1773 WP_002777199.1 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36040.60 Da        Isoelectric Point: 10.3664

>NTDB_id=356078 E5L67_RS07635 WP_162284669.1 1464773..1465717(-) (waaF) [Campylobacter coli strain 16SHKX65C]
MKIFIHLPTWLGDAVMASPALYGVYHHFKNAEFILYGSFVSTALFKEFPNAKIIVENKKSRYKQTLSLRKKLGKIDLALS
FRSALSSKIILHILKAKKRYFFNKYNFKEEHQVLKYLYFIENSLDIKAHSKDLKLPFKLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAQVALNFSKTHEILIFGAGKAEQELCDEIFHILKEKNVKVKNLCNKTTIKTLCQNIVFCDIFITNDS
GPMHIAAAYKTKTIAIFGPTKFTQTSPWQNQNAKLVHLNLSCMPCMQKTCPLKHHRCMKDLKPEKILEAIQNFT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=356078 E5L67_RS07635 WP_162284669.1 1464773..1465717(-) (waaF) [Campylobacter coli strain 16SHKX65C]
ATGAAAATTTTTATCCATCTTCCCACTTGGCTAGGCGATGCAGTCATGGCCTCACCTGCTTTATATGGAGTTTATCATCA
TTTTAAAAATGCTGAATTTATCCTTTATGGATCTTTTGTATCTACAGCGCTTTTTAAGGAATTTCCTAATGCTAAAATCA
TTGTAGAAAATAAAAAATCACGCTACAAACAAACCCTTTCTTTACGCAAAAAACTTGGAAAAATCGATCTAGCCCTTTCT
TTTAGATCAGCTCTTTCCTCTAAGATCATTTTGCATATCCTTAAGGCAAAAAAAAGATATTTTTTTAACAAGTATAATTT
CAAAGAAGAACATCAGGTTTTAAAATATCTTTATTTTATAGAAAATTCACTAGATATCAAAGCTCATTCTAAGGATTTAA
AACTTCCTTTTAAATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCCTAGGATTAAACCCTGGAGCG
AGTTTTGGAAGTGCTAAAAGATGGGATGCGAGCTATTTTGCACAAGTTGCTTTAAATTTTAGCAAAACTCATGAAATTTT
GATTTTTGGAGCAGGTAAAGCAGAACAAGAACTTTGCGATGAAATTTTTCATATACTAAAAGAAAAAAATGTCAAAGTAA
AAAATCTTTGCAATAAAACCACTATCAAAACCCTTTGTCAAAATATCGTTTTTTGTGATATTTTCATCACAAACGATAGC
GGCCCTATGCACATAGCTGCCGCTTATAAAACAAAAACCATAGCTATTTTTGGGCCTACTAAATTTACCCAAACCTCACC
TTGGCAAAATCAAAATGCAAAATTAGTACATCTAAATTTATCTTGCATGCCTTGCATGCAAAAAACCTGTCCTTTAAAAC
ATCATCGGTGCATGAAAGATTTAAAACCGGAAAAAATTTTAGAAGCAATCCAAAATTTTACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(108-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

83.067

99.682

0.828

Multiple sequence alignment