Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY32_RS10425 Genome accession   NZ_CP046828
Coordinates   346847..348076 (+) Length   409 a.a.
NCBI ID   WP_023667297.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2010V-1106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341847..353076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY32_RS10380 - 342148..342477 (-) 330 WP_005459127.1 DUF2500 domain-containing protein -
  GPY32_RS10385 - 342719..343087 (+) 369 WP_005489749.1 hypothetical protein -
  GPY32_RS10390 - 343150..343329 (-) 180 WP_005458791.1 hypothetical protein -
  GPY32_RS10400 - 344064..344684 (+) 621 WP_005481960.1 lysoplasmalogenase -
  GPY32_RS10405 - 344754..344975 (+) 222 WP_005455142.1 YecH family metal-binding protein -
  GPY32_RS10410 - 345095..345679 (+) 585 WP_005455144.1 YhgN family NAAT transporter -
  GPY32_RS10415 - 345732..345998 (-) 267 WP_005496405.1 DUF1145 domain-containing protein -
  GPY32_RS10420 rsmD 346024..346623 (-) 600 WP_005455148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GPY32_RS10425 pilA 346847..348076 (+) 1230 WP_023667297.1 signal recognition particle-docking protein FtsY Machinery gene
  GPY32_RS10430 ftsE 348099..348773 (+) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  GPY32_RS10435 ftsX 348763..349731 (+) 969 WP_005481956.1 permease-like cell division protein FtsX -
  GPY32_RS10440 rpoH 349907..350767 (+) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  GPY32_RS10445 glpE 351259..351579 (+) 321 WP_005496409.1 thiosulfate sulfurtransferase GlpE -
  GPY32_RS10450 glpG 351579..352421 (+) 843 WP_005496414.1 rhomboid family intramembrane serine protease GlpG -
  GPY32_RS10455 - 352481..352891 (-) 411 WP_005460376.1 flagellar basal body-associated protein FliL -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44538.15 Da        Isoelectric Point: 4.2803

>NTDB_id=355955 GPY32_RS10425 WP_023667297.1 346847..348076(+) (pilA) [Vibrio parahaemolyticus strain 2010V-1106]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVKEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEAEAEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTKII
NNLTEKASRGDLKDGEALYGLLKEEMAEILSKVEQPLDIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVMLAA
GDTFRAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKKID
DSAPHEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFGIPIRYIGVGEGIEDLRPFETQEF
IDALFSREE

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=355955 GPY32_RS10425 WP_023667297.1 346847..348076(+) (pilA) [Vibrio parahaemolyticus strain 2010V-1106]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGTCCAAAACCAAAAACTGA
AGAAACAGTAAAAGAAGAAGCAGTGGAAGCTCCTTCTGAAGAGCAGCAAACCGAAGAGGTTGCTGAGCAAGCTCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGCAGAAGCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAGCTTCTT
CGCTCGCCTTAAACGCAGCCTTAGCCGTACGAAAGCGAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTAAAAAAA
TCGATGACGACCTATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAAATCATC
AACAACCTGACAGAAAAAGCCTCTCGAGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGAAATGGC
GGAGATTCTATCTAAAGTAGAACAGCCTCTAGATATCGACAGTAGCAAAACACCTTATGTCATCTTGATGGTTGGTGTGA
ATGGTGTGGGTAAAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTAGCAGCT
GGCGATACTTTCCGAGCGGCCGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGCGCAGCA
TACTGGCGCAGACAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTCGATGTGGTTATCGCCG
ATACGGCGGGTCGTCTACAAAACAAAGCCAACCTAATGGAAGAGCTACGCAAGATTGTACGTGTGATGAAGAAAATTGAT
GATTCTGCACCACATGAAATCATGCTGACGCTGGATGCAGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACTTTTCAG
TGATGTAGCTCCTCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAAGGCGGCGTTATTTTCGCTATCGCTG
ATCAGTTTGGTATTCCAATTCGCTACATTGGTGTTGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAAGAGTTT
ATCGACGCTTTGTTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(125-186)

SRP54-type protein, GTPase domain (SRP54)

(206-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.958

93.888

0.46

Multiple sequence alignment