Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GP469_RS08090 Genome accession   NZ_CP046808
Coordinates   1618742..1619977 (+) Length   411 a.a.
NCBI ID   WP_069530257.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1146     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1613742..1624977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP469_RS08045 - 1614043..1614372 (-) 330 WP_005459127.1 DUF2500 domain-containing protein -
  GP469_RS08050 - 1614614..1614982 (+) 369 WP_005489749.1 hypothetical protein -
  GP469_RS08055 - 1615045..1615224 (-) 180 WP_005458791.1 hypothetical protein -
  GP469_RS08065 - 1615959..1616579 (+) 621 WP_005481960.1 lysoplasmalogenase -
  GP469_RS08070 - 1616649..1616870 (+) 222 WP_005455142.1 YecH family metal-binding protein -
  GP469_RS08075 - 1616990..1617574 (+) 585 WP_005455144.1 YhgN family NAAT transporter -
  GP469_RS08080 - 1617627..1617893 (-) 267 WP_005496405.1 DUF1145 domain-containing protein -
  GP469_RS08085 rsmD 1617919..1618518 (-) 600 WP_005455148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GP469_RS08090 pilA 1618742..1619977 (+) 1236 WP_069530257.1 signal recognition particle-docking protein FtsY Machinery gene
  GP469_RS08095 ftsE 1620000..1620674 (+) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  GP469_RS08100 ftsX 1620664..1621632 (+) 969 WP_005481956.1 permease-like cell division protein FtsX -
  GP469_RS08105 rpoH 1621808..1622668 (+) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  GP469_RS08110 glpE 1623160..1623480 (+) 321 WP_005496409.1 thiosulfate sulfurtransferase GlpE -
  GP469_RS08115 glpG 1623480..1624322 (+) 843 WP_005496414.1 rhomboid family intramembrane serine protease GlpG -
  GP469_RS08120 - 1624382..1624792 (-) 411 WP_005460376.1 flagellar basal body-associated protein FliL -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44738.34 Da        Isoelectric Point: 4.2657

>NTDB_id=355898 GP469_RS08090 WP_069530257.1 1618742..1619977(+) (pilA) [Vibrio parahaemolyticus strain 2013V-1146]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVKEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEAEAEAEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTK
IINNLTEKASRGDLKDGEALYGLLKEEMAEILSKVEQPLDIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVML
AAGDTFRAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKK
IDDSAPHEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFGIPIRYIGVGEGIEDLRPFETQ
EFIDALFSREE

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=355898 GP469_RS08090 WP_069530257.1 1618742..1619977(+) (pilA) [Vibrio parahaemolyticus strain 2013V-1146]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGTCCAAAACCAAAAACTGA
AGAAACAGTAAAAGAAGAAGCAGTGGAAGCTCCTTCTGAAGAGCAGCAAACCGAAGAGGTTGCTGAGCAAGCTCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGCAGAAGCAGAAGCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAG
CTTCTTCGCTCGCCTTAAACGCAGCCTTAGCCGTACGAAAGCGAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTA
AAAAAATCGATGACGACCTATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAA
ATCATCAACAACCTGACAGAAAAAGCCTCTCGAGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGA
AATGGCGGAGATTCTATCTAAAGTAGAACAGCCTCTAGATATCGACAGTAGCAAAACACCTTATGTCATCTTGATGGTTG
GTGTGAATGGTGTGGGTAAAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTA
GCAGCTGGCGATACTTTCCGAGCGGCCGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGC
GCAGCATACTGGCGCAGACAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTCGATGTGGTTA
TCGCCGATACGGCGGGTCGTCTACAAAACAAAGCCAACCTAATGGAAGAGCTACGCAAGATTGTACGTGTGATGAAGAAA
ATTGATGATTCTGCACCACATGAAATCATGCTGACGCTGGATGCAGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACT
TTTCAGTGATGTAGCTCCTCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAAGGCGGCGTTATTTTCGCTA
TCGCTGATCAGTTTGGTATTCCAATTCGCTACATTGGTGTTGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAA
GAGTTTATCGACGCTTTGTTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(127-188)

SRP54-type protein, GTPase domain (SRP54)

(208-407)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

93.674

0.457