Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY29_RS16515 Genome accession   NZ_CP046787
Coordinates   3289649..3291373 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain 2013V-1174     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284649..3296373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY29_RS16480 - 3284962..3285675 (-) 714 WP_029828005.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY29_RS16485 - 3285676..3286242 (-) 567 WP_031781777.1 type II secretion system protein -
  GPY29_RS16490 - 3286239..3286724 (-) 486 WP_021453808.1 type II secretion system protein -
  GPY29_RS16495 - 3286840..3287073 (-) 234 WP_017821316.1 hypothetical protein -
  GPY29_RS16500 - 3287151..3287657 (-) 507 WP_031781778.1 type II secretion system protein -
  GPY29_RS16505 - 3287952..3288413 (-) 462 WP_031781779.1 hypothetical protein -
  GPY29_RS16510 - 3288417..3289640 (-) 1224 WP_021484749.1 type II secretion system F family protein -
  GPY29_RS16515 pilB 3289649..3291373 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GPY29_RS16520 - 3291370..3292446 (-) 1077 WP_169600942.1 tetratricopeptide repeat protein -
  GPY29_RS16525 - 3292443..3293288 (-) 846 WP_031830056.1 AAA family ATPase -
  GPY29_RS16530 mshL 3293332..3294975 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  GPY29_RS16535 - 3294996..3295325 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GPY29_RS16540 gspM 3295318..3295968 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=355844 GPY29_RS16515 WP_005456306.1 3289649..3291373(-) (pilB) [Vibrio parahaemolyticus strain 2013V-1174]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355844 GPY29_RS16515 WP_005456306.1 3289649..3291373(-) (pilB) [Vibrio parahaemolyticus strain 2013V-1174]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTTCATGCTCGTCGTTTACGTGCCATGGTGGTGGCTCGCAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGCCAATCGATTGATAT
TCGTATGTCGACGTTACCAACTCAGTACGGTGAATCGGTTGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGATCAAGAGACGGTAGAG
ATTGGCTTACGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACTCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAATTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361