Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KAN01_RS15815 Genome accession   NZ_CP038816
Coordinates   3241615..3242415 (-) Length   266 a.a.
NCBI ID   WP_004710659.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain KAN01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3236615..3247415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN01_RS15795 (KAN01_15795) - 3237680..3238378 (-) 699 WP_002053293.1 YfgM family protein -
  KAN01_RS15800 (KAN01_15800) hisS 3238407..3239699 (-) 1293 WP_106479672.1 histidine--tRNA ligase -
  KAN01_RS15805 (KAN01_15805) ispG 3239696..3240811 (-) 1116 WP_032009832.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KAN01_RS15810 (KAN01_15810) - 3240833..3241624 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  KAN01_RS15815 (KAN01_15815) pilF 3241615..3242415 (-) 801 WP_004710659.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KAN01_RS15820 (KAN01_15820) rlmN 3242431..3243663 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KAN01_RS15825 (KAN01_15825) ndk 3243786..3244217 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  KAN01_RS15830 (KAN01_15830) iscX 3244331..3244531 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  KAN01_RS15835 (KAN01_15835) - 3244631..3245182 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  KAN01_RS15840 (KAN01_15840) - 3245207..3246505 (-) 1299 WP_079744809.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.44 Da        Isoelectric Point: 9.2307

>NTDB_id=355745 KAN01_RS15815 WP_004710659.1 3241615..3242415(-) (pilF) [Acinetobacter nosocomialis strain KAN01]
MSTPKLKIMLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=355745 KAN01_RS15815 WP_004710659.1 3241615..3242415(-) (pilF) [Acinetobacter nosocomialis strain KAN01]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCC
AACGCTTAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCCGCAGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAGCGTTCCCTTGACCAAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGCATTTTA
TTGCAACAAGAAGGTAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.496

100

0.985
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment