Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY17_RS23050 Genome accession   NZ_CP046782
Coordinates   2946238..2947962 (-) Length   574 a.a.
NCBI ID   WP_015297335.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2941238..2952962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY17_RS23015 - 2941574..2942287 (-) 714 WP_024701148.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY17_RS23020 - 2942287..2942886 (-) 600 WP_025507727.1 type II secretion system protein -
  GPY17_RS23025 - 2942883..2943362 (-) 480 WP_020841327.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY17_RS23030 - 2943478..2943711 (-) 234 WP_042772708.1 hypothetical protein -
  GPY17_RS23035 - 2943776..2944246 (-) 471 WP_021453864.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY17_RS23040 - 2944541..2945002 (-) 462 WP_005456302.1 MSHA biogenesis protein MshF -
  GPY17_RS23045 - 2945006..2946229 (-) 1224 WP_042772707.1 type II secretion system F family protein -
  GPY17_RS23050 pilB 2946238..2947962 (-) 1725 WP_015297335.1 GspE/PulE family protein Machinery gene
  GPY17_RS23055 - 2947959..2949026 (-) 1068 WP_175423148.1 tetratricopeptide repeat protein -
  GPY17_RS23060 - 2949023..2949868 (-) 846 WP_005456312.1 AAA family ATPase -
  GPY17_RS23065 mshL 2949912..2951555 (-) 1644 WP_042772705.1 pilus (MSHA type) biogenesis protein MshL -
  GPY17_RS23070 - 2951576..2951905 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GPY17_RS23075 gspM 2951898..2952548 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63902.21 Da        Isoelectric Point: 5.2324

>NTDB_id=355582 GPY17_RS23050 WP_015297335.1 2946238..2947962(-) (pilB) [Vibrio parahaemolyticus strain 2013V-1244]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLSRLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355582 GPY17_RS23050 WP_015297335.1 2946238..2947962(-) (pilB) [Vibrio parahaemolyticus strain 2013V-1244]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGATCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTAACCGTTGTAAAACTGGTCAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCTTGCGTTTGCGCCAGC
GTGTCGACGGTGTGCTACATGAAACCATTCTGAATGAAGTGAACATCGCTTCAGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGTCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAAATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGCGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCAGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCATAAAGGAGCTGGTTGTCAAAACTGTAACTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGCGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.387

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361

Multiple sequence alignment