Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY25_RS02070 Genome accession   NZ_CP046779
Coordinates   403423..404652 (-) Length   409 a.a.
NCBI ID   WP_023667297.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2014V-1066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398423..409652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY25_RS02040 - 398608..399018 (+) 411 WP_005460376.1 flagellar basal body-associated protein FliL -
  GPY25_RS02045 glpG 399078..399920 (-) 843 WP_005496414.1 rhomboid family intramembrane serine protease GlpG -
  GPY25_RS02050 glpE 399920..400240 (-) 321 WP_005496409.1 thiosulfate sulfurtransferase GlpE -
  GPY25_RS02055 rpoH 400732..401592 (-) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  GPY25_RS02060 ftsX 401768..402736 (-) 969 WP_005481956.1 permease-like cell division protein FtsX -
  GPY25_RS02065 ftsE 402726..403400 (-) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  GPY25_RS02070 pilA 403423..404652 (-) 1230 WP_023667297.1 signal recognition particle-docking protein FtsY Machinery gene
  GPY25_RS02075 rsmD 404876..405475 (+) 600 WP_005455148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GPY25_RS02080 - 405501..405767 (+) 267 WP_005496405.1 DUF1145 domain-containing protein -
  GPY25_RS02085 - 405820..406404 (-) 585 WP_005455144.1 YhgN family NAAT transporter -
  GPY25_RS02090 - 406524..406745 (-) 222 WP_005455142.1 YecH family metal-binding protein -
  GPY25_RS02095 - 406815..407435 (-) 621 WP_005481960.1 lysoplasmalogenase -
  GPY25_RS02105 - 408170..408349 (+) 180 WP_005458791.1 hypothetical protein -
  GPY25_RS02110 - 408412..408780 (-) 369 WP_005489749.1 hypothetical protein -
  GPY25_RS02115 - 409022..409351 (+) 330 WP_005459127.1 DUF2500 domain-containing protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44538.15 Da        Isoelectric Point: 4.2803

>NTDB_id=355436 GPY25_RS02070 WP_023667297.1 403423..404652(-) (pilA) [Vibrio parahaemolyticus strain 2014V-1066]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVKEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEAEAEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTKII
NNLTEKASRGDLKDGEALYGLLKEEMAEILSKVEQPLDIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVMLAA
GDTFRAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKKID
DSAPHEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFGIPIRYIGVGEGIEDLRPFETQEF
IDALFSREE

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=355436 GPY25_RS02070 WP_023667297.1 403423..404652(-) (pilA) [Vibrio parahaemolyticus strain 2014V-1066]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGTCCAAAACCAAAAACTGA
AGAAACAGTAAAAGAAGAAGCAGTGGAAGCTCCTTCTGAAGAGCAGCAAACCGAAGAGGTTGCTGAGCAAGCTCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGCAGAAGCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAGCTTCTT
CGCTCGCCTTAAACGCAGCCTTAGCCGTACGAAAGCGAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTAAAAAAA
TCGATGACGACCTATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAAATCATC
AACAACCTGACAGAAAAAGCCTCTCGAGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGAAATGGC
GGAGATTCTATCTAAAGTAGAACAGCCTCTAGATATCGACAGTAGCAAAACACCTTATGTCATCTTGATGGTTGGTGTGA
ATGGTGTGGGTAAAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTAGCAGCT
GGCGATACTTTCCGAGCGGCCGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGCGCAGCA
TACTGGCGCAGACAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTCGATGTGGTTATCGCCG
ATACGGCGGGTCGTCTACAAAACAAAGCCAACCTAATGGAAGAGCTACGCAAGATTGTACGTGTGATGAAGAAAATTGAT
GATTCTGCACCACATGAAATCATGCTGACGCTGGATGCAGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACTTTTCAG
TGATGTAGCTCCTCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAAGGCGGCGTTATTTTCGCTATCGCTG
ATCAGTTTGGTATTCCAATTCGCTACATTGGTGTTGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAAGAGTTT
ATCGACGCTTTGTTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(125-186)

SRP54-type protein, GTPase domain (SRP54)

(206-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.958

93.888

0.46

Multiple sequence alignment