Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY46_RS24770 Genome accession   NZ_CP046776
Coordinates   3219482..3220705 (-) Length   407 a.a.
NCBI ID   WP_005491145.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain AM43962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3214482..3225705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY46_RS24740 - 3214667..3215077 (+) 411 WP_005460376.1 flagellar basal body-associated protein FliL -
  GPY46_RS24745 glpG 3215137..3215979 (-) 843 WP_005496414.1 rhomboid family intramembrane serine protease GlpG -
  GPY46_RS24750 glpE 3215979..3216299 (-) 321 WP_005460380.1 thiosulfate sulfurtransferase GlpE -
  GPY46_RS24755 rpoH 3216791..3217651 (-) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  GPY46_RS24760 ftsX 3217827..3218795 (-) 969 WP_005481956.1 permease-like cell division protein FtsX -
  GPY46_RS24765 ftsE 3218785..3219459 (-) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  GPY46_RS24770 pilA 3219482..3220705 (-) 1224 WP_005491145.1 signal recognition particle-docking protein FtsY Machinery gene
  GPY46_RS24775 rsmD 3220929..3221528 (+) 600 WP_005455148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GPY46_RS24780 - 3221554..3221820 (+) 267 WP_158128049.1 DUF1145 domain-containing protein -
  GPY46_RS24785 - 3221874..3222458 (-) 585 WP_005455144.1 YhgN family NAAT transporter -
  GPY46_RS24790 - 3222578..3222799 (-) 222 WP_015297472.1 YecH family metal-binding protein -
  GPY46_RS24795 - 3222869..3223489 (-) 621 WP_005481960.1 lysoplasmalogenase -
  GPY46_RS24805 - 3224224..3224403 (+) 180 WP_005458791.1 hypothetical protein -
  GPY46_RS24810 - 3224466..3224834 (-) 369 WP_005489749.1 hypothetical protein -
  GPY46_RS24815 - 3225076..3225405 (+) 330 WP_015297474.1 DUF2500 domain-containing protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44354.94 Da        Isoelectric Point: 4.2729

>NTDB_id=355327 GPY46_RS24770 WP_005491145.1 3219482..3220705(-) (pilA) [Vibrio parahaemolyticus strain AM43962]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVTEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEAEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTKIINN
LTEKASRGDLKDGEALYGLLKEEMAEILSKVEQPLEIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVMLAAGD
TFRAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKKIDDS
APHEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFSIPIRYIGVGEGIEDLRPFETQEFID
ALFSREE

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=355327 GPY46_RS24770 WP_005491145.1 3219482..3220705(-) (pilA) [Vibrio parahaemolyticus strain AM43962]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGCCCAAAACCAAAAACTGA
AGAAACAGTAACAGAAGAAGCAGTGGAAGCGCCTTCTGAAGAGCAGCAAACCGAAGAGGTTGCTGAGCAAGCTCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAGCTTCTTCGCTCG
CCTTAAACGCAGCCTTAGCCGTACGAAAGCGAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTAAAAAAATCGATG
ACGACCTATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAAATCATCAACAAC
CTGACAGAAAAAGCCTCTCGTGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGAAATGGCGGAGAT
TCTATCTAAAGTAGAACAGCCTCTAGAGATCGACAGCAGCAAAACACCTTACGTCATCTTGATGGTTGGTGTGAATGGTG
TGGGTAAAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTAGCGGCTGGCGAT
ACTTTCCGAGCGGCTGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGCGCAGCATACTGG
CGCAGACAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTCGATGTGGTTATCGCCGATACGG
CGGGTCGTCTACAAAACAAAGCCAACCTAATGGAAGAGCTACGCAAGATTGTACGTGTGATGAAGAAAATTGATGATTCT
GCACCACATGAAATCATGCTGACGCTGGATGCGGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACTTTTCAGTGATGT
AGCTCCTCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAAGGCGGCGTTATTTTCGCTATCGCTGATCAGT
TTAGTATTCCAATTCGCTACATTGGTGTTGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAAGAGTTTATCGAC
GCTTTGTTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(123-184)

SRP54-type protein, GTPase domain (SRP54)

(204-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.072

92.629

0.455

Multiple sequence alignment