Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY46_RS23245 Genome accession   NZ_CP046776
Coordinates   2911118..2912842 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain AM43962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2906118..2917842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY46_RS23210 - 2906422..2907135 (-) 714 WP_025504117.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY46_RS23215 - 2907135..2907734 (-) 600 WP_025504118.1 type II secretion system protein -
  GPY46_RS23220 - 2907731..2908210 (-) 480 WP_025504119.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY46_RS23225 - 2908326..2908559 (-) 234 WP_025504120.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY46_RS23230 - 2908623..2909126 (-) 504 WP_025504122.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY46_RS23235 - 2909421..2909882 (-) 462 WP_021823195.1 hypothetical protein -
  GPY46_RS23240 - 2909886..2911109 (-) 1224 WP_025544351.1 type II secretion system F family protein -
  GPY46_RS23245 pilB 2911118..2912842 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GPY46_RS23250 - 2912839..2913909 (-) 1071 WP_180697518.1 tetratricopeptide repeat protein -
  GPY46_RS23255 - 2913906..2914751 (-) 846 WP_015297337.1 AAA family ATPase -
  GPY46_RS23260 mshL 2914795..2916438 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  GPY46_RS23265 - 2916459..2916788 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GPY46_RS23270 gspM 2916781..2917431 (-) 651 WP_020841336.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=355315 GPY46_RS23245 WP_005456306.1 2911118..2912842(-) (pilB) [Vibrio parahaemolyticus strain AM43962]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355315 GPY46_RS23245 WP_005456306.1 2911118..2912842(-) (pilB) [Vibrio parahaemolyticus strain AM43962]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCAGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTTTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCCGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGATGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTTGTAATGCGTCTGCTTAACCAGTCGTCAGGCTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCACGCTTGCGTCGTCAGTTATCTCGCCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGCATCACGCAAGTACAGATCAACAGCAAGATCGACCTGACATTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGATCAAGAGACGGTAGAG
ATTGGCTTACGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTTCACAAAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGATATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGATAAGCTGCGCGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCGATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361

Multiple sequence alignment