Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E5D16_RS09025 Genome accession   NZ_CP038642
Coordinates   1769394..1770767 (-) Length   457 a.a.
NCBI ID   WP_003723898.1    Uniprot ID   L8DQ47
Organism   Listeria monocytogenes strain N12-0935     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1764394..1775767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5D16_RS08995 (E5D16_08995) epsC 1764497..1765111 (-) 615 WP_012951129.1 serine O-acetyltransferase EpsC -
  E5D16_RS09005 (E5D16_09005) gltX 1765509..1766984 (-) 1476 WP_003732826.1 glutamate--tRNA ligase -
  E5D16_RS09010 (E5D16_09010) ispF 1767003..1767476 (-) 474 WP_003732825.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E5D16_RS09015 (E5D16_09015) ispD 1767469..1768167 (-) 699 WP_003723900.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  E5D16_RS09020 (E5D16_09020) - 1768187..1769260 (-) 1074 WP_003723899.1 PIN/TRAM domain-containing protein -
  E5D16_RS09025 (E5D16_09025) radA 1769394..1770767 (-) 1374 WP_003723898.1 DNA repair protein RadA Machinery gene
  E5D16_RS09030 (E5D16_09030) - 1770913..1773375 (-) 2463 WP_031671564.1 ATP-dependent Clp protease ATP-binding subunit -
  E5D16_RS09035 (E5D16_09035) - 1773404..1774426 (-) 1023 WP_009925721.1 protein arginine kinase -
  E5D16_RS09040 (E5D16_09040) - 1774423..1774941 (-) 519 WP_003723895.1 UvrB/UvrC motif-containing protein -
  E5D16_RS09045 (E5D16_09045) - 1774954..1775412 (-) 459 WP_003723894.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49991.63 Da        Isoelectric Point: 7.9613

>NTDB_id=355266 E5D16_RS09025 WP_003723898.1 1769394..1770767(-) (radA) [Listeria monocytogenes strain N12-0935]
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=355266 E5D16_RS09025 WP_003723898.1 1769394..1770767(-) (radA) [Listeria monocytogenes strain N12-0935]
ATGGCTAAAGCAAAAAGGACAACCAAATTTGTGTGTCAGGCATGTGGATATGAATCGGCAAAATGGATGGGAAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTAGAAGCCTTAGAACCGTCAAAAAAATCACGCTCAGCTTTCAATCATACAGGAG
AGCCTTCAAAAGCGACTCCAATTACTCAAATAGCAAGTGAAACAGAAAAACGAGTTGAAACAAATATGCCCGAGTTAAAT
AGAGTTCTTGGTGGCGGTGTGGTTCCTGGGTCTATGGTACTTGTCGGAGGAGACCCTGGTATTGGTAAATCAACATTGTT
ATTACAAGTATCAGCGCAACTCACGCTTACAAATAAAAAAGTATTGTATATCTCAGGAGAAGAATCAATCAAACAAACGA
AGCTACGGGCAGAACGCTTACAAGTTTCGGGAGATAATTTATATGTGTATGCAGAAACAAATTTAGAAGCAGTTCAAGAA
ACGATTGATTTTGTAAAACCTGATTTTGTAGTCATTGACTCTATTCAGACTGTTTATCATCCTGATGTTACAAGTGCAGC
AGGGAGTGTTTCTCAAGTTAGGGAATGTACAGCGACATTGATGCGAATTGCTAAAATGCAAAACATCGCTATCTTTATTG
TTGGGCATGTAACTAAGGAAGGTGCTATTGCGGGACCGCGCCTATTAGAACATATGGTTGATACTGTACTCTATTTTGAA
GGGGAGCGTCACCATGCTTACCGGATTTTGCGTGCAGTGAAAAACCGTTTTGGTTCCACTAACGAAATGGGTATATTTGA
AATGCGAGATGTAGGACTTGTTGAAGTTGCTAATCCTTCCGAGGTATTCTTGGAAGAACGTCTTGAAGGCGCTTCAGGCT
CAACGGTTGTAGCTTCTATGGAAGGAACTCGCCCCGTCCTTGTGGAAATACAAGCGCTTGTTTCGCCAACGATGTTCGGT
AATGCTAAGCGAATGGCGACGGGAATAGACTACAACAAAGTTTCGCTAATTATGGCTGTTTTAGAAAAGCGAGTAGGTTT
AATGTTGCAAAACCAAGATGCTTATTTGAAAGCGGCGGGCGGGGTTAAATTGGATGAGCCTGCAGTGGATTTGGCGGTTG
CAGTCAGTGTTGCATCTAGTTATCGTGATAAACCCACAAGAAGTACGGACTGCTTTATTGGGGAACTTGGACTTACAGGT
GAAATTCGTCGTGTAGCAAGAATTGAACAACGCGTACAAGAAGCAGCAAAACTTGGCTTTAAGCGAATTTTTATTCCTAA
AAATAACGAAGGTAATTGGAAAATACCGAAAGACGTGCAAGTGGTTGGGGTGGAAACGATTGGAGAAGCTTTGAAGAAAG
CTTTACCAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L8DQ47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.421

99.781

0.683
  radA Streptococcus pneumoniae Rx1

65.265

98.906

0.646
  radA Streptococcus pneumoniae D39

65.265

98.906

0.646
  radA Streptococcus pneumoniae R6

65.265

98.906

0.646
  radA Streptococcus pneumoniae TIGR4

65.265

98.906

0.646
  radA Streptococcus mitis NCTC 12261

65.265

98.906

0.646
  radA Streptococcus mitis SK321

65.044

98.906

0.643

Multiple sequence alignment