Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E0W60_RS12500 Genome accession   NZ_CP038635
Coordinates   359502..360980 (-) Length   492 a.a.
NCBI ID   WP_133093641.1    Uniprot ID   A0A4V1BYJ6
Organism   Cupriavidus oxalaticus strain X32     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354502..365980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0W60_RS12470 (E0W60_12470) - 354930..355598 (-) 669 WP_133093638.1 LON peptidase substrate-binding domain-containing protein -
  E0W60_RS12475 (E0W60_12475) rapZ 355703..356590 (-) 888 WP_135704362.1 RNase adapter RapZ -
  E0W60_RS12480 (E0W60_12480) - 356717..357049 (-) 333 WP_133093640.1 PsiF family protein -
  E0W60_RS12485 (E0W60_12485) hprK 357228..358199 (-) 972 WP_063236682.1 HPr(Ser) kinase/phosphatase -
  E0W60_RS12490 (E0W60_12490) ptsN 358279..358734 (-) 456 WP_029046777.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E0W60_RS12495 (E0W60_12495) raiA 359103..359453 (-) 351 WP_029046778.1 ribosome-associated translation inhibitor RaiA -
  E0W60_RS12500 (E0W60_12500) rpoN/rpoN1 359502..360980 (-) 1479 WP_133093641.1 RNA polymerase factor sigma-54 Machinery gene
  E0W60_RS12505 (E0W60_12505) lptB 361136..361936 (-) 801 WP_133093642.1 LPS export ABC transporter ATP-binding protein -
  E0W60_RS12510 (E0W60_12510) lptA 362005..362652 (-) 648 WP_133093643.1 lipopolysaccharide transport periplasmic protein LptA -
  E0W60_RS12515 (E0W60_12515) lptC 362792..363421 (-) 630 WP_135704364.1 LPS export ABC transporter periplasmic protein LptC -
  E0W60_RS12520 (E0W60_12520) - 363465..364448 (-) 984 WP_135704366.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54810.15 Da        Isoelectric Point: 4.8667

>NTDB_id=355229 E0W60_RS12500 WP_133093641.1 359502..360980(-) (rpoN/rpoN1) [Cupriavidus oxalaticus strain X32]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARAEGAGDDDSYGDSNSGDDYGGDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISVRDKGLAIF
LIESLDDDGYLSATLEEICAELPEELEFEIEEVQAMLTLLQSFDPPGVGARNAAECLGLQLRRLQHPQRELALNIVHNHL
ELLAVRDYTRLKKALQVDEPALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSGWIAQLNPDVMPRLRINDMYA
QILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=355229 E0W60_RS12500 WP_133093641.1 359502..360980(-) (rpoN/rpoN1) [Cupriavidus oxalaticus strain X32]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCACCTGGCACTGACGCCGCAACTGCAGCAGTCGATCAGGCTGTTGCA
GCTTTCGACGCTGGAATTGCAGCAGGAAGTCGAACAGGCGCTCACGGAAAACCCCCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCCGCCGATGGCTCGGTCAACCTGCAGAGCGCGCCCGCGCCGGCACCGGCCGAACCGCAA
GGCAACGGCGAGGCGCGCGCCGAGGGCGCGGGCGATGACGACAGCTACGGCGACAGCAACAGCGGCGACGACTATGGCGG
CGACTGGAGCCTCGACGATTTCGCGCGCCGCCCGCAAGGCGACGAAGACGAGAAGACGCCGATGCAGCTGCGTGAGGCCG
AGCCCACGCTGCGCGAATACCTGATGGAACAGCTCACGCCGCTGAAGATCTCGGTGCGCGACAAGGGCCTGGCCATCTTC
CTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGACGCTCGAAGAGATCTGCGCCGAACTGCCCGAAGAACTCGA
ATTCGAGATCGAGGAAGTACAAGCGATGCTGACGCTGCTGCAGAGCTTCGACCCGCCGGGCGTCGGCGCGCGCAACGCGG
CCGAGTGCCTGGGCCTGCAATTGCGCCGGCTGCAGCATCCGCAGCGCGAGTTGGCGCTGAACATCGTGCATAACCACCTC
GAGCTGCTGGCCGTGCGCGACTACACGCGCCTGAAAAAAGCGCTGCAGGTAGACGAGCCTGCACTCAAGGCCGCGCATGA
ACTGATCCGCTCGCTGGCACCCTACCCGGGCCACGCATACAGCCGCCCCGAAGCCGACTTCGTCGTGCCCGATGTGTTCG
TGCGCAAGAGCGGCAGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGCGGCTGCGCATCAATGACATGTACGCG
CAGATCCTGCGCGGCGCCAAGGGCGAGTCGGGTACCGCAGGCCTGCAGCAGAAACTGCAGGAAGCGCGCTGGCTGATCAA
GAATATCCAGCAAAGGTTCGACACGATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCACGCACG
GCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATCTCCCGGGTG
ACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGAGAC
CGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCCTTT
CCGACAGTCGCATCGCCGAACTGCTGGGTGAGCAAGGCTTCGTTGTCGCACGACGCACCGTAGCCAAATATCGCGAAGCC
CTGAAGATCCCCGCAGTGAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V1BYJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.641

100

0.811

Multiple sequence alignment