Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY19_RS04235 Genome accession   NZ_CP046763
Coordinates   874584..876308 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain 2012AW-0353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869584..881308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY19_RS04200 - 869947..870660 (-) 714 WP_025631907.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY19_RS04205 - 870661..871227 (-) 567 WP_046265379.1 type II secretion system protein -
  GPY19_RS04210 - 871224..871709 (-) 486 WP_046268072.1 type II secretion system protein -
  GPY19_RS04215 - 871825..872058 (-) 234 WP_005388453.1 MSHA biogenesis protein MshA -
  GPY19_RS04220 - 872127..872591 (-) 465 WP_025552739.1 type II secretion system protein -
  GPY19_RS04225 - 872887..873348 (-) 462 WP_024701144.1 hypothetical protein -
  GPY19_RS04230 - 873352..874575 (-) 1224 WP_015297334.1 type II secretion system F family protein -
  GPY19_RS04235 pilB 874584..876308 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GPY19_RS04240 - 876305..877381 (-) 1077 WP_029854408.1 tetratricopeptide repeat protein -
  GPY19_RS04245 - 877378..878223 (-) 846 WP_005497278.1 AAA family ATPase -
  GPY19_RS04250 mshL 878267..879910 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  GPY19_RS04255 - 879931..880260 (-) 330 WP_062905567.1 hypothetical protein -
  GPY19_RS04260 gspM 880253..880903 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=355182 GPY19_RS04235 WP_005456306.1 874584..876308(-) (pilB) [Vibrio parahaemolyticus strain 2012AW-0353]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355182 GPY19_RS04235 WP_005456306.1 874584..876308(-) (pilB) [Vibrio parahaemolyticus strain 2012AW-0353]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGGCGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTTCATGCTCGTCGTTTACGTGCCATGGTGGTGGCTCGCAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGCCAATCGATTGATAT
TCGTATGTCGACGTTACCAACTCAGTACGGTGAATCGGTTGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAGCCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAAATCGACCTGACATTTT
CACGCGTGTTACGCACCTTCTTACGCCAAGACCCAGACATTATTTTGATTGGTGAGATGCGCGACCAAGAGACGGTAGAG
ATTGGCTTGAGAGCAGCACTAACCGGCCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGATATGGGGGCGCCAGGCTATCTTGTTGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTCCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCTGGTTGTCAAAACTGTAATTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCACTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361