Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY27_RS01085 Genome accession   NZ_CP046761
Coordinates   216497..218221 (+) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain AM46865     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211497..223221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY27_RS01060 gspM 211902..212552 (+) 651 WP_005497276.1 type II secretion system protein GspM -
  GPY27_RS01065 - 212545..212874 (+) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GPY27_RS01070 mshL 212895..214538 (+) 1644 WP_140095431.1 pilus (MSHA type) biogenesis protein MshL -
  GPY27_RS01075 - 214582..215427 (+) 846 WP_005497278.1 AAA family ATPase -
  GPY27_RS01080 - 215424..216500 (+) 1077 WP_180784183.1 tetratricopeptide repeat protein -
  GPY27_RS01085 pilB 216497..218221 (+) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GPY27_RS01090 - 218230..219453 (+) 1224 WP_021484749.1 type II secretion system F family protein -
  GPY27_RS01095 - 219457..219918 (+) 462 WP_140095429.1 MSHA biogenesis protein MshF -
  GPY27_RS01100 - 220213..220704 (+) 492 WP_020835336.1 type II secretion system protein -
  GPY27_RS01105 - 220777..221010 (+) 234 WP_005481019.1 hypothetical protein -
  GPY27_RS01110 - 221126..221605 (+) 480 WP_042780812.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY27_RS01115 - 221602..222201 (+) 600 WP_025509946.1 type II secretion system protein -
  GPY27_RS01120 - 222201..222914 (+) 714 WP_025611258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=355079 GPY27_RS01085 WP_005456306.1 216497..218221(+) (pilB) [Vibrio parahaemolyticus strain AM46865]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355079 GPY27_RS01085 WP_005456306.1 216497..218221(+) (pilB) [Vibrio parahaemolyticus strain AM46865]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCAGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCCATGGTGGTGGCTCGCAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTCGAGTTCATCATAGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCCGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCCGACAGTGAAGAAGTTACCGTTGTAAAACTGGTTAATTC
GATGTTTGAGGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTACATGAAACCATTCTGAATGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGCTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAAATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACATTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGATATCATTTTGATTGGTGAGATGCGCGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGCTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACTCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGATATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361