Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E4664_RS14250 Genome accession   NZ_CP038500
Coordinates   2890641..2891441 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CIAT758     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2885641..2896441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4664_RS14230 (E4664_14230) - 2886706..2887404 (-) 699 WP_000058787.1 YfgM family protein -
  E4664_RS14235 (E4664_14235) hisS 2887433..2888725 (-) 1293 WP_000095261.1 histidine--tRNA ligase -
  E4664_RS14240 (E4664_14240) ispG 2888722..2889837 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E4664_RS14245 (E4664_14245) - 2889859..2890650 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  E4664_RS14250 (E4664_14250) pilF 2890641..2891441 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E4664_RS14255 (E4664_14255) rlmN 2891457..2892689 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E4664_RS14260 (E4664_14260) ndk 2892812..2893243 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  E4664_RS14265 (E4664_14265) iscX 2893357..2893557 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  E4664_RS14270 (E4664_14270) - 2893657..2894208 (-) 552 WP_000669568.1 phosphatase PAP2 family protein -
  E4664_RS14275 (E4664_14275) - 2894233..2895531 (-) 1299 WP_086225874.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=354858 E4664_RS14250 WP_004738086.1 2890641..2891441(-) (pilF) [Acinetobacter baumannii strain CIAT758]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=354858 E4664_RS14250 WP_004738086.1 2890641..2891441(-) (pilF) [Acinetobacter baumannii strain CIAT758]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCTTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACTTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment