Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPW72_RS17245 Genome accession   NZ_CP046744
Coordinates   2549549..2550133 (+) Length   194 a.a.
NCBI ID   WP_000902745.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3569-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544549..2555133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW72_RS17220 - 2544711..2545655 (-) 945 WP_000800334.1 AAA family ATPase -
  GPW72_RS17225 - 2545682..2547685 (-) 2004 WP_001895336.1 transposase domain-containing protein -
  GPW72_RS17230 - 2547697..2547921 (-) 225 WP_000373567.1 helix-turn-helix transcriptional regulator -
  GPW72_RS17235 - 2548074..2548811 (+) 738 WP_000005494.1 XRE family transcriptional regulator -
  GPW72_RS17240 pilM 2549080..2549559 (+) 480 Protein_2215 type IV pilus assembly protein PilM -
  GPW72_RS17245 pilN 2549549..2550133 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  GPW72_RS17250 pilO 2550126..2550716 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW72_RS17255 pilP 2550706..2551224 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  GPW72_RS17260 pilQ 2551250..2552965 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  GPW72_RS17265 aroK 2553152..2553676 (+) 525 WP_000818616.1 shikimate kinase AroK -
  GPW72_RS17270 aroB 2553704..2554789 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 21964.42 Da        Isoelectric Point: 9.9503

>NTDB_id=354711 GPW72_RS17245 WP_000902745.1 2549549..2550133(+) (pilN) [Vibrio cholerae strain 3569-08]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQAGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=354711 GPW72_RS17245 WP_000902745.1 2549549..2550133(+) (pilN) [Vibrio cholerae strain 3569-08]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCAGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAACAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGCGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

100

100

1
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552