Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EBI04_RS12590 Genome accession   NZ_CP038452
Coordinates   2361681..2362595 (-) Length   304 a.a.
NCBI ID   WP_135257742.1    Uniprot ID   -
Organism   Thermus caldilimi strain YIM 78456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2356681..2367595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBI04_RS12580 - 2357137..2358267 (-) 1131 WP_135257740.1 hypothetical protein -
  EBI04_RS12585 - 2358308..2361340 (-) 3033 WP_135257741.1 Eco57I restriction-modification methylase domain-containing protein -
  EBI04_RS12590 pilA 2361681..2362595 (-) 915 WP_135257742.1 signal recognition particle-docking protein FtsY Machinery gene
  EBI04_RS12595 - 2362638..2363078 (-) 441 WP_135257743.1 hypothetical protein -
  EBI04_RS12600 gltX 2363110..2364528 (-) 1419 WP_135257744.1 glutamate--tRNA ligase -
  EBI04_RS12605 lptB 2364583..2365311 (+) 729 WP_135257745.1 LPS export ABC transporter ATP-binding protein -
  EBI04_RS12610 - 2365315..2366700 (+) 1386 WP_135257746.1 DUF3084 domain-containing protein -
  EBI04_RS12615 prmC 2366608..2367465 (-) 858 WP_135257747.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32897.15 Da        Isoelectric Point: 7.3622

>NTDB_id=354489 EBI04_RS12590 WP_135257742.1 2361681..2362595(-) (pilA) [Thermus caldilimi strain YIM 78456]
MGFFDRLKAGLAKTRETLLKAIPWGGSPEEVLEELEMALLAADVGLEATEELLAEVRASGRKDLKEAVKEKLVQMLEPDE
RRATLRKLGFRPQTPKPVEPQGHVVLVVGVNGVGKTTTIAKLGRYYRNLGKKVMFCAGDTFRAAGGAQLSEWGKRLDIPV
IQGPEGADPAALAFDAASARKARGYDLLLVDTAGRLHTKHNLMEELKKVKRAIAKADPEEPKEVWLVLDAVTGQNGLEQA
KRFHEAVGLTGVIVTKLDGTAKGGVLIPIVRTLKVPIRFIGVGEGPDDLQPFDAEAFVEALLEG

Nucleotide


Download         Length: 915 bp        

>NTDB_id=354489 EBI04_RS12590 WP_135257742.1 2361681..2362595(-) (pilA) [Thermus caldilimi strain YIM 78456]
ATGGGCTTCTTTGACCGACTGAAAGCCGGCCTCGCCAAGACGCGAGAAACCCTCCTCAAGGCCATCCCCTGGGGCGGAAG
CCCCGAGGAGGTCCTGGAGGAGCTGGAAATGGCCCTCCTCGCCGCCGACGTGGGCCTCGAGGCCACCGAGGAGCTCCTGG
CCGAGGTACGGGCTTCGGGACGGAAAGACCTGAAGGAGGCCGTAAAGGAGAAGCTCGTCCAGATGCTGGAGCCGGACGAG
CGCCGGGCCACCTTGAGGAAACTCGGCTTCCGCCCGCAAACCCCTAAGCCCGTGGAACCCCAGGGCCACGTGGTTCTGGT
GGTGGGGGTAAACGGGGTGGGCAAGACCACCACCATCGCCAAGCTGGGACGCTACTACCGGAACCTGGGCAAGAAGGTGA
TGTTCTGCGCCGGGGACACCTTCCGCGCGGCCGGGGGAGCCCAGCTTTCCGAGTGGGGGAAACGCTTGGACATCCCCGTG
ATCCAAGGGCCAGAAGGGGCCGACCCCGCCGCTTTGGCCTTTGATGCGGCAAGCGCCCGGAAAGCCAGGGGCTACGACCT
CCTCCTGGTGGACACCGCAGGCCGCCTCCACACCAAGCACAACCTGATGGAGGAGCTGAAGAAGGTGAAGCGGGCCATCG
CCAAGGCCGATCCCGAGGAGCCCAAGGAGGTCTGGCTGGTGCTGGACGCCGTCACCGGGCAAAACGGCCTGGAGCAGGCC
AAGCGCTTCCACGAGGCGGTGGGGCTCACCGGGGTCATCGTCACCAAGCTGGACGGCACCGCCAAGGGCGGAGTCCTCAT
TCCCATCGTGCGCACCCTTAAGGTGCCCATCCGCTTCATCGGGGTGGGGGAAGGCCCAGACGACCTCCAGCCCTTTGACG
CCGAGGCCTTTGTGGAGGCGTTGCTAGAGGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(29-70)

SRP54-type protein, GTPase domain (SRP54)

(104-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.682

100

0.513

Multiple sequence alignment