Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   EBI04_RS00530 Genome accession   NZ_CP038452
Coordinates   104388..104858 (-) Length   156 a.a.
NCBI ID   WP_135255591.1    Uniprot ID   -
Organism   Thermus caldilimi strain YIM 78456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99388..109858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBI04_RS13715 - 100258..100620 (-) 363 WP_135255586.1 type II secretion system protein -
  EBI04_RS00510 - 100849..102579 (-) 1731 WP_135255587.1 hypothetical protein -
  EBI04_RS00515 - 102590..103339 (-) 750 WP_135255588.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EBI04_RS00520 - 103336..103863 (-) 528 WP_135255589.1 type II secretion system protein -
  EBI04_RS00525 - 103860..104333 (-) 474 WP_135255590.1 GspH/FimT family pseudopilin -
  EBI04_RS00530 pilA/pilA1 104388..104858 (-) 471 WP_135255591.1 GspH/FimT family protein Machinery gene
  EBI04_RS00535 - 104929..106119 (+) 1191 WP_135255592.1 thiolase family protein -
  EBI04_RS00540 - 106116..106424 (-) 309 WP_135255593.1 DUF503 domain-containing protein -
  EBI04_RS00545 - 106444..108909 (-) 2466 WP_135255594.1 outer membrane protein assembly factor -
  EBI04_RS00550 - 108955..109782 (+) 828 WP_135255595.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16891.60 Da        Isoelectric Point: 11.1539

>NTDB_id=354470 EBI04_RS00530 WP_135255591.1 104388..104858(-) (pilA/pilA1) [Thermus caldilimi strain YIM 78456]
MRREAFTLLELLVILAILGVLLGLGLPLLSPNRLALDAAARSLAAQVTRARLEAIRRNAFAGLHVFTEGAGGYLIFVDHD
GDRRYAPGEEVQVVRFGEGDWARVRLDQGQSRLGNLPLLFDPRGVPTKPITATIALASGSATRKVIVSQQGRARLE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=354470 EBI04_RS00530 WP_135255591.1 104388..104858(-) (pilA/pilA1) [Thermus caldilimi strain YIM 78456]
ATGCGCAGGGAAGCTTTTACCCTTCTAGAGCTTCTGGTTATCCTGGCAATCCTGGGCGTGCTTCTGGGCCTGGGGCTTCC
CCTCCTTTCCCCCAACCGCCTGGCCCTGGACGCTGCCGCCCGCTCCCTGGCGGCCCAGGTGACGAGGGCCCGGCTGGAAG
CCATTCGGCGTAACGCCTTCGCCGGGCTCCACGTCTTCACCGAGGGGGCAGGGGGCTACCTGATCTTCGTGGACCATGAT
GGCGACCGCCGCTATGCCCCAGGGGAGGAGGTCCAGGTGGTGCGCTTCGGGGAGGGGGACTGGGCCCGGGTGCGCCTGGA
CCAGGGGCAAAGCCGCCTGGGGAATCTGCCCCTCCTTTTCGACCCCCGCGGAGTACCCACCAAGCCCATCACCGCCACCA
TAGCTCTCGCTTCCGGAAGCGCAACCCGCAAGGTGATCGTAAGCCAGCAGGGCCGGGCCCGCCTAGAGTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

76.378

81.41

0.622

Multiple sequence alignment