Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E4R25_RS02715 Genome accession   NZ_CP038451
Coordinates   543763..545175 (+) Length   470 a.a.
NCBI ID   WP_002817898.1    Uniprot ID   A0A483B8S8
Organism   Oenococcus oeni strain SD-2a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 538763..550175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4R25_RS02675 (E4R25_02680) - 539272..539940 (+) 669 WP_002822411.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  E4R25_RS02680 (E4R25_02685) - 540115..540393 (+) 279 WP_002817904.1 hypothetical protein -
  E4R25_RS02685 (E4R25_02690) - 540399..541004 (+) 606 WP_002817903.1 DNA-3-methyladenine glycosylase I -
  E4R25_RS02690 (E4R25_02695) - 541001..542050 (-) 1050 WP_002822409.1 AI-2E family transporter -
  E4R25_RS02695 (E4R25_02700) - 542141..542434 (-) 294 WP_002822408.1 GNAT family N-acetyltransferase -
  E4R25_RS02700 (E4R25_02705) - 542538..542729 (+) 192 WP_002817901.1 hypothetical protein -
  E4R25_RS02705 (E4R25_02710) - 542802..543257 (+) 456 WP_002822407.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  E4R25_RS02710 (E4R25_02715) - 543257..543727 (+) 471 WP_002818140.1 NUDIX hydrolase -
  E4R25_RS02715 (E4R25_02720) radA 543763..545175 (+) 1413 WP_002817898.1 DNA repair protein RadA Machinery gene
  E4R25_RS02720 (E4R25_02725) pgmB 545194..545865 (+) 672 WP_002822406.1 beta-phosphoglucomutase -
  E4R25_RS02725 (E4R25_02730) rdgB 545962..546576 (+) 615 WP_002817896.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E4R25_RS02730 (E4R25_02735) gltX 546599..548095 (+) 1497 WP_002822405.1 glutamate--tRNA ligase -
  E4R25_RS02735 (E4R25_02740) - 548217..549035 (+) 819 WP_002817894.1 phosphoribosyltransferase -
  E4R25_RS02740 (E4R25_02745) - 549076..549633 (+) 558 WP_002822404.1 hypothetical protein -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 50699.13 Da        Isoelectric Point: 6.5132

>NTDB_id=354459 E4R25_RS02715 WP_002817898.1 543763..545175(+) (radA) [Oenococcus oeni strain SD-2a]
MAKAKTQFVCSNCGYTSARYLGRCPNCGQWNTLIEEKIVPEAVVNRKARISFDGRVAKPKKISEISGNELPRTRTGLREL
NRVLGGGIVPGSLILIGGDPGIGKSTLMLQVSGQLAENGGSVLYVSGEESDNQIKLRADRLGVGSDSFIVYPETDMEQIK
SIVQQIEPDYLVIDSVQTMQEPDLQSPIGSVAQIREVTADLMQIAKTNAITVFIVGHVTKDGSIAGPKILEHMVDTVLYF
EGDANYKYRILRTVKNRFGSTNELGIFEMRDHGLREVANPSEIFLEERLAGATGSAITASMEGSRPILVEVQALVAPTVF
GNAQRVTTGVDRNRVAQILAVLEKHANLLLQNQDAHVRITGGVKIDEPAADLAIALAVASSYHEKATNASDVFLGEVGLG
GEVRSISLIEDRLKEIAKLGFKRAIVSKNNLAGIDLPKGLQVIGVSTLEEALKLGLDLNRASKQKGFADL

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=354459 E4R25_RS02715 WP_002817898.1 543763..545175(+) (radA) [Oenococcus oeni strain SD-2a]
ATGGCAAAAGCAAAAACACAATTTGTTTGTTCCAACTGTGGCTATACCAGTGCTCGCTATCTTGGGCGCTGTCCGAATTG
CGGCCAATGGAATACATTAATAGAGGAAAAAATTGTTCCCGAAGCAGTTGTAAACCGAAAAGCTCGAATCAGTTTCGACG
GGCGGGTTGCCAAACCGAAAAAGATTTCTGAAATCTCTGGTAATGAATTGCCTAGAACAAGAACTGGATTAAGGGAATTA
AATCGCGTCCTCGGTGGCGGGATCGTCCCGGGAAGCTTAATTTTGATTGGCGGCGATCCAGGAATAGGGAAATCGACTTT
AATGCTTCAGGTTTCTGGTCAGTTGGCCGAAAATGGCGGAAGTGTTCTTTATGTTTCCGGAGAAGAGTCGGATAATCAGA
TTAAATTGCGTGCTGATCGACTTGGCGTTGGTTCGGATTCTTTTATTGTTTATCCGGAAACGGATATGGAACAAATTAAA
TCAATTGTCCAACAAATCGAACCGGATTATTTGGTAATTGATTCGGTTCAGACAATGCAGGAACCTGATCTGCAGTCGCC
AATTGGCTCGGTTGCCCAAATACGAGAAGTGACGGCCGACTTAATGCAGATAGCCAAAACGAATGCGATTACGGTTTTCA
TTGTTGGGCATGTTACTAAAGATGGATCGATCGCCGGTCCGAAAATTCTCGAGCACATGGTTGATACGGTTCTGTATTTT
GAAGGGGATGCCAATTATAAGTACCGTATCCTTCGAACTGTTAAAAATCGTTTTGGATCGACCAATGAACTTGGTATCTT
TGAAATGCGTGATCATGGTTTACGAGAAGTAGCCAATCCATCGGAGATCTTTCTCGAAGAGCGTTTGGCCGGCGCAACCG
GATCGGCAATTACAGCCAGTATGGAGGGCAGTCGTCCGATTTTAGTCGAAGTGCAGGCTTTGGTCGCACCAACGGTTTTC
GGTAATGCTCAGCGTGTGACAACCGGCGTTGATCGAAATCGCGTTGCTCAGATTTTAGCCGTTTTGGAAAAACATGCCAA
TCTGCTTTTACAAAATCAGGATGCCCATGTTCGAATTACCGGAGGCGTTAAAATCGATGAGCCGGCAGCTGATTTGGCAA
TTGCTTTAGCCGTTGCCAGTTCTTACCATGAAAAAGCAACTAATGCCTCCGATGTTTTTCTTGGTGAAGTAGGTTTGGGA
GGAGAAGTCCGTTCAATTTCTCTGATTGAAGATCGTTTGAAAGAGATTGCCAAACTTGGATTTAAACGGGCAATTGTTTC
AAAAAATAATTTGGCAGGAATCGATTTGCCAAAAGGTCTGCAGGTGATTGGTGTTTCTACCTTGGAAGAAGCATTGAAAT
TAGGCCTCGATTTGAATAGGGCTTCCAAACAGAAAGGTTTTGCTGATTTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(367-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(76-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A483B8S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

61.589

96.383

0.594
  radA Streptococcus pneumoniae Rx1

61.589

96.383

0.594
  radA Streptococcus pneumoniae D39

61.589

96.383

0.594
  radA Streptococcus pneumoniae TIGR4

61.589

96.383

0.594
  radA Bacillus subtilis subsp. subtilis str. 168

60.832

97.234

0.591
  radA Streptococcus mitis NCTC 12261

61.369

96.383

0.591
  radA Streptococcus mitis SK321

61.148

96.383

0.589

Multiple sequence alignment