Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E4186_RS02050 Genome accession   NZ_CP038444
Coordinates   423740..425179 (+) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media strain T5-8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418740..430179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4186_RS02020 (E4186_02045) mlaF 419318..420118 (-) 801 WP_025328535.1 phospholipid ABC transporter ATP-binding protein MlaF -
  E4186_RS02025 (E4186_02050) - 420325..421320 (+) 996 WP_025328534.1 KpsF/GutQ family sugar-phosphate isomerase -
  E4186_RS02030 (E4186_02055) kdsC 421320..421874 (+) 555 WP_042649051.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  E4186_RS02035 (E4186_02060) lptC 421871..422431 (+) 561 WP_042649050.1 LPS export ABC transporter periplasmic protein LptC -
  E4186_RS02040 (E4186_02065) lptA 422418..422936 (+) 519 WP_042649049.1 lipopolysaccharide transport periplasmic protein LptA -
  E4186_RS02045 (E4186_02070) lptB 422949..423674 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  E4186_RS02050 (E4186_02075) rpoN/rpoN1 423740..425179 (+) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  E4186_RS02055 (E4186_02080) hpf 425200..425487 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  E4186_RS02060 (E4186_02085) ptsN 425490..425936 (+) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E4186_RS02065 (E4186_02090) rapZ 425977..426843 (+) 867 WP_041204881.1 RNase adapter RapZ -
  E4186_RS02070 (E4186_02095) npr 426864..427136 (+) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  E4186_RS22825 (E4186_02100) - 427155..428200 (-) 1046 Protein_394 PTS sugar transporter subunit IIA -
  E4186_RS02080 (E4186_02105) degS 428315..429436 (-) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=354375 E4186_RS02050 WP_041204880.1 423740..425179(+) (rpoN/rpoN1) [Aeromonas media strain T5-8]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=354375 E4186_RS02050 WP_041204880.1 423740..425179(+) (rpoN/rpoN1) [Aeromonas media strain T5-8]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACTACCTGGGATGAGGTCTATTCCGCTGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment