Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E4185_RS08385 Genome accession   NZ_CP038443
Coordinates   1757465..1758904 (+) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media strain R1-18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1752465..1763904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4185_RS08355 (E4185_08420) mlaF 1753044..1753844 (-) 801 WP_171273118.1 phospholipid ABC transporter ATP-binding protein MlaF -
  E4185_RS08360 (E4185_08425) - 1754051..1755046 (+) 996 WP_041204878.1 KpsF/GutQ family sugar-phosphate isomerase -
  E4185_RS08365 (E4185_08430) kdsC 1755046..1755599 (+) 554 Protein_1603 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  E4185_RS08370 (E4185_08435) lptC 1755596..1756156 (+) 561 WP_171273119.1 LPS export ABC transporter periplasmic protein LptC -
  E4185_RS08375 (E4185_08440) lptA 1756143..1756661 (+) 519 WP_171274316.1 lipopolysaccharide transport periplasmic protein LptA -
  E4185_RS08380 (E4185_08445) lptB 1756674..1757399 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  E4185_RS08385 (E4185_08450) rpoN/rpoN1 1757465..1758904 (+) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  E4185_RS08390 (E4185_08455) hpf 1758925..1759212 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  E4185_RS08395 (E4185_08460) ptsN 1759215..1759661 (+) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E4185_RS08400 (E4185_08465) rapZ 1759702..1760568 (+) 867 WP_171273120.1 RNase adapter RapZ -
  E4185_RS08405 (E4185_08470) npr 1760589..1760861 (+) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  E4185_RS08410 (E4185_08475) - 1760880..1761925 (-) 1046 Protein_1612 PTS sugar transporter subunit IIA -
  E4185_RS08415 (E4185_08480) degS 1762040..1763161 (-) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=354360 E4185_RS08385 WP_041204880.1 1757465..1758904(+) (rpoN/rpoN1) [Aeromonas media strain R1-18]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=354360 E4185_RS08385 WP_041204880.1 1757465..1758904(+) (rpoN/rpoN1) [Aeromonas media strain R1-18]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAACCCCAGGACGCCAGTCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACTACCTGGGATGAGGTCTATTCCGCCGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCTGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCTT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGACTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGTGCCCTTATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment