Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E4184_RS20010 Genome accession   NZ_CP038441
Coordinates   4317525..4318964 (-) Length   479 a.a.
NCBI ID   WP_171276963.1    Uniprot ID   -
Organism   Aeromonas media strain T0.1-19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4312525..4323964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4184_RS19980 (E4184_20140) - 4312798..4313538 (+) 741 WP_171276961.1 KDGP aldolase family protein -
  E4184_RS19985 (E4184_20145) - 4313645..4315549 (+) 1905 WP_171276962.1 PRD domain-containing protein -
  E4184_RS19990 (E4184_20150) npr 4315568..4315840 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  E4184_RS19995 (E4184_20155) rapZ 4315861..4316727 (-) 867 WP_139438909.1 RNase adapter RapZ -
  E4184_RS20000 (E4184_20160) ptsN 4316768..4317214 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E4184_RS20005 (E4184_20165) hpf 4317217..4317504 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  E4184_RS20010 (E4184_20170) rpoN/rpoN1 4317525..4318964 (-) 1440 WP_171276963.1 RNA polymerase factor sigma-54 Machinery gene
  E4184_RS20015 (E4184_20175) lptB 4319030..4319755 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  E4184_RS20020 (E4184_20180) lptA 4319768..4320286 (-) 519 WP_042649049.1 lipopolysaccharide transport periplasmic protein LptA -
  E4184_RS20025 (E4184_20185) lptC 4320273..4320833 (-) 561 WP_042649050.1 LPS export ABC transporter periplasmic protein LptC -
  E4184_RS20030 (E4184_20190) kdsC 4320830..4321384 (-) 555 WP_042649051.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  E4184_RS20035 (E4184_20195) - 4321384..4322379 (-) 996 WP_025328534.1 KpsF/GutQ family sugar-phosphate isomerase -
  E4184_RS20040 (E4184_20200) mlaF 4322586..4323386 (+) 801 WP_025328535.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53778.73 Da        Isoelectric Point: 4.4152

>NTDB_id=354341 E4184_RS20010 WP_171276963.1 4317525..4318964(-) (rpoN/rpoN1) [Aeromonas media strain T0.1-19]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSDEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGTKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=354341 E4184_RS20010 WP_171276963.1 4317525..4318964(-) (rpoN/rpoN1) [Aeromonas media strain T0.1-19]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCAGGAAACCAGCCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGCGACGCCATGGCGCAGGACAAGATGCCG
GATGAACTGCCGGTGGACACCACCTGGGATGAGGTCTACTCCGCCGGTACGGCCCAGAGCGCCGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCTTGACGCCGTTCA
GCGATCTGGATGCCGCCATCGCCCTCGCCATCATCGATGCCATCGATGAGTCCGGTTACCTGACCGTCTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGCGATGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATAGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGATCTGCTCGGCAACCGCGACTACCGCACCCTGGCACGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGCAATGGCATCAT
CCAGAGTGAATCGCAATACGTGATCCCGGATGTCTCCGTGGTCAAGAAGGGGACTAAGTGGGTGGTTGAGCTCAACCCGG
ATTCGGCGCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCACGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATTCGCTCCCATCTGCAGGAAGCCAAATGGTTCATCAAGAGCCTGGAGAGCCGCAACGACACCCTACTCAAGGTGGCCAG
CTGCATAGTCGAGCAGCAGCAGGGTTTCTTCGAATATGGTGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.471

100

0.495

Multiple sequence alignment