Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E4T63_RS24130 Genome accession   NZ_CP038438
Coordinates   5228485..5229852 (-) Length   455 a.a.
NCBI ID   WP_007963764.1    Uniprot ID   A0A423MHI7
Organism   Pseudomonas fluorescens strain LBUM677     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5223485..5234852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS24100 (E4T63_24100) - 5223577..5224287 (+) 711 WP_135296602.1 GntR family transcriptional regulator -
  E4T63_RS24105 (E4T63_24105) yjiA 5224404..5225375 (-) 972 WP_135296603.1 GTPase -
  E4T63_RS24115 (E4T63_24115) - 5225528..5225725 (-) 198 WP_003228401.1 YbdD/YjiX family protein -
  E4T63_RS24120 (E4T63_24120) - 5225741..5227807 (-) 2067 WP_007963766.1 carbon starvation CstA family protein -
  E4T63_RS24125 (E4T63_24125) - 5227967..5228335 (+) 369 WP_098965715.1 PilZ domain-containing protein -
  E4T63_RS24130 (E4T63_24130) radA 5228485..5229852 (-) 1368 WP_007963764.1 DNA repair protein RadA Machinery gene
  E4T63_RS24135 (E4T63_24135) - 5229896..5230432 (-) 537 WP_135296604.1 ankyrin repeat domain-containing protein -
  E4T63_RS24140 (E4T63_24140) katB 5230493..5232034 (-) 1542 WP_098965718.1 catalase KatB -
  E4T63_RS24145 (E4T63_24145) mscL 5232320..5232733 (+) 414 WP_135296605.1 large-conductance mechanosensitive channel protein MscL -
  E4T63_RS24150 (E4T63_24150) - 5232776..5233552 (-) 777 WP_135296606.1 ferredoxin--NADP reductase -
  E4T63_RS24155 (E4T63_24155) - 5233885..5234613 (+) 729 WP_135296607.1 autoinducer binding domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48757.25 Da        Isoelectric Point: 6.8987

>NTDB_id=354294 E4T63_RS24130 WP_007963764.1 5228485..5229852(-) (radA) [Pseudomonas fluorescens strain LBUM677]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMIESGGATAPTGRTGWAGQQAQIKTLAEVSIEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=354294 E4T63_RS24130 WP_007963764.1 5228485..5229852(-) (radA) [Pseudomonas fluorescens strain LBUM677]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCGCAACCTTTCCCAAGTGGGCAGGCCAGTGCGGCGAGTG
CGGCGCCTGGAACACCCTGACCGAAACCATGATCGAAAGCGGCGGGGCCACGGCGCCCACCGGGCGTACCGGCTGGGCCG
GGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCATCGAGGAGATTCCGCGCTTCTCCACCGCGTCCGGTGAGCTG
GATCGGGTTTTGGGTGGCGGTCTGGTCGACGGCTCGGTGGTGCTGATCGGTGGTGACCCGGGCATCGGCAAGTCGACGAT
TCTGTTACAGACCCTGTGCAACCTCGCCAAGAGCATGCCGGCGCTGTACGTCACCGGCGAAGAATCCCAGCAGCAGGTGG
CGATGCGCGCCCGGCGTCTGGGGCTGCCCCAGGATCAACTGCGGGTGATGACCGAAACCTGCATCGAAACCATCATTGCC
ACCGCCCGTCAGGAAAAACCCAAGGTGATGGTGATCGACTCGATCCAGACCATTTTCACCGAACAGCTGCAATCAGCGCC
GGGTGGCGTCTCGCAGGTACGTGAGAGCGCGGCGTTGCTGGTGCGTTATGCCAAGCAGAGTGGCACGGCGATCTTCCTCG
TCGGTCACGTCACCAAGGAAGGCGCGCTCGCCGGCCCGCGGGTGCTGGAGCACATGGTCGATACCGTGCTGTATTTCGAA
GGCGAATCCGATGGCCGTTTGCGTCTGTTGCGGGCGGTGAAAAACCGTTTCGGTGCGGTCAACGAACTCGGTGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCTTCGGCGATTTTTCTCACCCGTGCCCAGGAAGAAGTCCCGGGCA
GTGTGGTCATGGCCACCTGGGAAGGCACCCGGCCGATGCTGGTGGAAGTGCAGGCGCTGGTGGATGACAGTCATCTGGCC
AATCCACGGCGCGTGACGTTGGGCCTTGATCAGAACCGATTGGCGATGTTGTTGGCGGTATTGCACCGTCACGGTGGTAT
TCCGACTCACGACCAGGACGTGTTCCTCAACGTGGTCGGCGGGGTGAAGGTGTTGGAGACCGCATCGGATCTGGCATTGA
TGGCGGCGGTGATGTCGAGCCTGCGCAACCGGCCTTTGCCGCATGACCTGCTGGTGTTTGGTGAAGTGGGCTTGTCCGGC
GAAGTGCGTCCGGTGCCGAGTGGGCAGGAGCGTTTGAAGGAAGCGGCCAAGCATGGCTTCAAACGGGCGATCGTGCCCAA
GGGCAATGCGCCGAAGGAGGCGCCGCCGGGGTTACAGATCATTGCCGTGACCCGCCTGGAGCAGGCGCTCGACGCATTAT
TCGAGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(81-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A423MHI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.453

100

0.497
  radA Streptococcus pneumoniae Rx1

46.304

100

0.468
  radA Streptococcus pneumoniae R6

46.304

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.468
  radA Streptococcus pneumoniae D39

46.304

100

0.468
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464

Multiple sequence alignment