Detailed information NTG0354230

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GPL39_RS00860
• Genome accession: NZ_CP046716
• Coordinates: 178013..178996 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain T16R
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 173013..183996

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPL39_RS00840 (GPL39_00840)	dppA	174156..175763 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
GPL39_RS25015	-	175797..175907 (-)	111	Protein_168	hypothetical protein	-
GPL39_RS00850 (GPL39_00850)	dppB	176071..177090 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GPL39_RS00855 (GPL39_00855)	dppC	177100..178002 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GPL39_RS00860 (GPL39_00860)	amiE	178013..178996 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GPL39_RS00865 (GPL39_00865)	dppF	178993..179997 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GPL39_RS00870 (GPL39_00870)	yhjV	180027..181298 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
GPL39_RS00875 (GPL39_00875)	ldrD	181774..181881 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
GPL39_RS00880 (GPL39_00880)	bcsG	181968..183647 (-)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
GPL39_RS00885 (GPL39_00885)	bcsF	183644..183835 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=354230 GPL39_RS00860 WP_001196486.1 178013..178996(+) (amiE) [Escherichia coli strain T16R]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=354230 GPL39_RS00860 WP_001196486.1 178013..178996(+) (amiE) [Escherichia coli strain T16R]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404