Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GO005_RS13025 Genome accession   NZ_CP046592
Coordinates   2535673..2536083 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain TR21     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2530673..2541083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO005_RS12995 (GO005_12975) - 2530785..2530925 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GO005_RS13000 (GO005_12980) yqeF 2531231..2531962 (-) 732 WP_032722145.1 SGNH/GDSL hydrolase family protein -
  GO005_RS13005 (GO005_12985) cwlH 2532214..2532966 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GO005_RS13010 (GO005_12990) yqeD 2533153..2533779 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  GO005_RS13015 (GO005_12995) gnd 2533798..2534666 (-) 869 Protein_2531 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GO005_RS13020 (GO005_13000) yqeB 2534918..2535640 (+) 723 WP_010886572.1 hypothetical protein -
  GO005_RS13025 (GO005_13005) nucA/comI 2535673..2536083 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GO005_RS13030 (GO005_13010) - 2536279..2536626 (+) 348 Protein_2534 sigma-70 family RNA polymerase sigma factor -
  GO005_RS13035 (GO005_13015) spoIVCA 2536657..2538117 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  GO005_RS21270 - 2538075..2538253 (-) 179 Protein_2536 hypothetical protein -
  GO005_RS13040 (GO005_13020) arsC 2538608..2539027 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GO005_RS13045 (GO005_13025) acr3 2539039..2540079 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  GO005_RS13050 (GO005_13030) arsK 2540102..2540542 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GO005_RS13055 (GO005_13035) arsR 2540601..2540918 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=353774 GO005_RS13025 WP_009967785.1 2535673..2536083(-) (nucA/comI) [Bacillus subtilis strain TR21]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=353774 GO005_RS13025 WP_009967785.1 2535673..2536083(-) (nucA/comI) [Bacillus subtilis strain TR21]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment