Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GO004_RS00630 Genome accession   NZ_CP046591
Coordinates   100056..100466 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain R31     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 95056..105466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO004_RS00600 (GO004_00590) arsR 95219..95536 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  GO004_RS00605 (GO004_00595) arsK 95597..96037 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GO004_RS00610 (GO004_00600) acr3 96060..97100 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  GO004_RS00615 (GO004_00605) arsC 97112..97531 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GO004_RS21740 - 97886..98064 (+) 179 Protein_125 hypothetical protein -
  GO004_RS00620 (GO004_00610) spoIVCA 98022..99482 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  GO004_RS00625 (GO004_00615) - 99513..99860 (-) 348 Protein_127 sigma-70 family RNA polymerase sigma factor -
  GO004_RS00630 (GO004_00620) nucA/comI 100056..100466 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GO004_RS00635 (GO004_00625) yqeB 100499..101221 (-) 723 WP_010886572.1 hypothetical protein -
  GO004_RS00640 (GO004_00630) gnd 101473..102366 (+) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GO004_RS00645 (GO004_00635) yqeD 102385..103011 (-) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  GO004_RS00650 (GO004_00640) cwlH 103198..103950 (+) 753 WP_015714296.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GO004_RS00655 (GO004_00645) yqeF 104203..104934 (+) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  GO004_RS00660 (GO004_00650) - 105240..105380 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=353527 GO004_RS00630 WP_009967785.1 100056..100466(+) (nucA/comI) [Bacillus subtilis strain R31]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=353527 GO004_RS00630 WP_009967785.1 100056..100466(+) (nucA/comI) [Bacillus subtilis strain R31]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529