Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GOE21_RS18995 Genome accession   NZ_CP046539
Coordinates   3883932..3885425 (+) Length   497 a.a.
NCBI ID   WP_001040630.1    Uniprot ID   A7ZT00
Organism   Escherichia coli strain FHI_NMBU_10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3878932..3890425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOE21_RS18965 (GOE21_18960) tusA 3879179..3879424 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  GOE21_RS18970 (GOE21_18965) zntA 3879526..3881724 (-) 2199 WP_000106522.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GOE21_RS18975 (GOE21_18970) yhhN 3881798..3882424 (-) 627 WP_000964718.1 lysoplasmalogenase -
  GOE21_RS18980 (GOE21_18975) yhhM 3882565..3882924 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  GOE21_RS18985 (GOE21_18980) yhhL 3882927..3883196 (-) 270 WP_001295207.1 DUF1145 family protein -
  GOE21_RS18990 (GOE21_18985) rsmD 3883186..3883782 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GOE21_RS18995 (GOE21_18990) pilA 3883932..3885425 (+) 1494 WP_001040630.1 signal recognition particle-docking protein FtsY Machinery gene
  GOE21_RS19000 (GOE21_18995) ftsE 3885428..3886096 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GOE21_RS19005 (GOE21_19000) ftsX 3886089..3887147 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  GOE21_RS19010 (GOE21_19005) rpoH 3887392..3888246 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GOE21_RS19015 (GOE21_19010) livJ 3888517..3889620 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GOE21_RS19020 (GOE21_19015) panM 3889819..3890202 (-) 384 WP_000778805.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54530.33 Da        Isoelectric Point: 4.1560

>NTDB_id=353190 GOE21_RS18995 WP_001040630.1 3883932..3885425(+) (pilA) [Escherichia coli strain FHI_NMBU_10]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=353190 GOE21_RS18995 WP_001040630.1 3883932..3885425(+) (pilA) [Escherichia coli strain FHI_NMBU_10]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGTAA
CAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGGCGC
GTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.654

81.489

0.388