Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GOE21_RS12625 Genome accession   NZ_CP046539
Coordinates   2536818..2537258 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain FHI_NMBU_10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531818..2542258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOE21_RS12605 (GOE21_12615) aroP 2532816..2534189 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GOE21_RS12610 (GOE21_12620) ampE 2534232..2535086 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GOE21_RS12615 (GOE21_12625) ampD 2535083..2535634 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GOE21_RS12620 (GOE21_12630) nadC 2535722..2536615 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GOE21_RS12625 (GOE21_12635) pilA 2536818..2537258 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GOE21_RS12630 (GOE21_12640) pilB 2537268..2538653 (+) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  GOE21_RS12635 (GOE21_12645) hofC 2538643..2539845 (+) 1203 WP_000157234.1 protein transport protein HofC -
  GOE21_RS12640 (GOE21_12650) guaC 2539880..2540923 (-) 1044 WP_001217338.1 GMP reductase -
  GOE21_RS12645 - 2541079..2541123 (-) 45 WP_120795372.1 protein YacM -
  GOE21_RS12650 (GOE21_12655) coaE 2541148..2541768 (+) 621 WP_086257641.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=353161 GOE21_RS12625 WP_000360904.1 2536818..2537258(+) (pilA) [Escherichia coli strain FHI_NMBU_10]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=353161 GOE21_RS12625 WP_000360904.1 2536818..2537258(+) (pilA) [Escherichia coli strain FHI_NMBU_10]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363