Detailed information NTG0353074

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E4T55_RS14240
• Genome accession: NZ_CP038273
• Coordinates: 3080447..3081511 (+)
• Length: 354 a.a.
• NCBI ID: WP_058501754.1
• Uniprot ID: A0A0W0VUE9
• Organism: Legionella israelensis strain Bercovier 4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3075447..3086511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4T55_RS14230 (E4T55_14230)	-	3076631..3078994 (-)	2364	WP_058501756.1	penicillin-binding protein 1A	-
E4T55_RS14235 (E4T55_14235)	-	3079185..3080189 (-)	1005	WP_058501755.1	hypothetical protein	-
E4T55_RS14240 (E4T55_14240)	pilM	3080447..3081511 (+)	1065	WP_058501754.1	type IV pilus assembly protein PilM	Machinery gene
E4T55_RS14245 (E4T55_14245)	pilN	3081515..3082087 (+)	573	WP_058501753.1	PilN domain-containing protein	Machinery gene
E4T55_RS14250 (E4T55_14250)	pilO	3082099..3082698 (+)	600	WP_058501752.1	type 4a pilus biogenesis protein PilO	Machinery gene
E4T55_RS14255 (E4T55_14255)	pilQ	3082829..3083974 (+)	1146	Protein_2802	type IV pilus secretin PilQ	-
E4T55_RS14260 (E4T55_14260)	aroK	3084056..3084577 (+)	522	WP_172460999.1	shikimate kinase AroK	-
E4T55_RS14265 (E4T55_14265)	aroB	3084564..3085670 (+)	1107	WP_058501750.1	3-dehydroquinate synthase	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 39008.06 Da
• Isoelectric Point: 4.5440

>NTDB_id=353074 E4T55_RS14240 WP_058501754.1 3080447..3081511(+) (pilM) [Legionella israelensis strain Bercovier 4]
MLKLLKPKKKSILGIDVSSTSVKILEISGEAPNYQLNAYASELLPEKAVEANVIKDVDAVAGCIKRATSRAKFSTKLAAA
AVPDSAVINKVIQINEGLNDEEMEELVVMEADKYIPYPIDEINIDFEILGPSAKNSTMLDVLIVASRAENVSNRAEVLTQ
AGLEAKIVDVESYAVERAVQLIAPDLPAEGVDKVIAVIDVGANYTHLFVLHGMKTVFSREEEFGARLLIESLAQRYEMTY
EQALEAYMQNQLLEDYKETVLEPFQEMMLLQVKRTLQFFYSTSNHGFVDHILLAGGLARQEGLVDLIQQQLGVPTTIANP
FKHMQLGKMLNSEVLNKEAPALLVACGLALRSIE

Nucleotide

• Length: 1065 bp

>NTDB_id=353074 E4T55_RS14240 WP_058501754.1 3080447..3081511(+) (pilM) [Legionella israelensis strain Bercovier 4]
GTGCTCAAATTGCTTAAGCCTAAAAAGAAATCAATATTGGGTATAGACGTCAGCTCAACTTCCGTTAAGATCCTGGAGAT
TTCTGGTGAGGCTCCGAATTATCAGTTGAATGCTTATGCCAGCGAACTTTTGCCCGAAAAAGCGGTAGAAGCGAATGTAA
TTAAGGATGTTGATGCGGTAGCTGGATGCATTAAAAGAGCAACTTCTCGCGCAAAATTTTCCACTAAACTAGCGGCTGCA
GCGGTACCTGACTCCGCTGTCATTAATAAGGTAATACAAATCAATGAAGGCTTAAATGACGAGGAGATGGAAGAACTGGT
CGTCATGGAAGCTGATAAATATATCCCTTACCCTATAGATGAAATTAATATTGATTTTGAAATCCTTGGGCCTTCAGCAA
AAAATTCCACCATGCTTGATGTGTTGATTGTCGCATCCCGAGCGGAAAATGTAAGTAATCGAGCTGAGGTACTTACCCAG
GCAGGTCTTGAGGCTAAAATAGTTGATGTTGAATCTTATGCTGTCGAACGGGCAGTACAGTTAATAGCCCCTGATCTTCC
GGCGGAAGGGGTAGATAAAGTTATTGCTGTGATTGATGTAGGAGCCAACTACACGCATTTATTTGTTTTGCATGGAATGA
AAACGGTGTTTTCCCGTGAAGAGGAATTTGGTGCCCGGCTTCTCATTGAATCATTGGCACAGCGATATGAAATGACCTAT
GAACAGGCTCTGGAAGCCTACATGCAAAATCAGCTGCTCGAAGATTACAAGGAAACGGTACTGGAGCCTTTTCAGGAAAT
GATGCTGTTACAGGTTAAACGTACCTTGCAGTTTTTTTATTCTACCAGCAACCATGGTTTTGTGGATCATATTCTTCTTG
CAGGTGGATTGGCACGGCAGGAAGGACTGGTTGATCTTATCCAGCAACAATTAGGAGTACCTACGACCATTGCAAATCCC
TTTAAACATATGCAATTGGGTAAAATGCTTAACTCGGAAGTCCTCAATAAGGAGGCGCCTGCATTGCTTGTTGCTTGTGG
ATTGGCACTGAGGTCAATAGAGTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A0W0VUE9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	66.384	100	0.664
comM	Acinetobacter baylyi ADP1	42.254	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter nosocomialis M2	42.09	100	0.421