Detailed information NTG0352989

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: GN312_RS04265
• Genome accession: NZ_CP046527
• Coordinates: 732534..733526 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain RM19259
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 727534..738526

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GN312_RS04240 (GN312_04240)	pphB	728896..729552 (+)	657	WP_001141350.1	protein-serine/threonine phosphatase	-
GN312_RS04245 (GN312_04245)	-	729593..729829 (-)	237	WP_000562982.1	non-oxidative hydroxyarylic acid decarboxylases subunit D	-
GN312_RS04250 (GN312_04250)	-	729840..731267 (-)	1428	WP_000863205.1	non-oxidative hydroxyarylic acid decarboxylases subunit C	-
GN312_RS04255 (GN312_04255)	-	731267..731860 (-)	594	WP_000767724.1	non-oxidative hydroxyarylic acid decarboxylases subunit B	-
GN312_RS04260 (GN312_04260)	-	732007..732414 (+)	408	WP_000175365.1	MarR family transcriptional regulator	-
GN312_RS04265 (GN312_04265)	rpoS	732534..733526 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
GN312_RS04270 (GN312_04270)	nlpD	733589..734635 (-)	1047	WP_306344721.1	murein hydrolase activator NlpD	-
GN312_RS04275 (GN312_04275)	pcm	734820..735446 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
GN312_RS04280 (GN312_04280)	surE	735440..736201 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
GN312_RS04285 (GN312_04285)	truD	736182..737231 (-)	1050	WP_000568934.1	tRNA pseudouridine(13) synthase TruD	-
GN312_RS04290 (GN312_04290)	ispF	737228..737707 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
GN312_RS04295 (GN312_04295)	ispD	737707..738417 (-)	711	WP_000246158.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=352989 GN312_RS04265 WP_000081550.1 732534..733526(-) (rpoS) [Escherichia coli strain RM19259]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=352989 GN312_RS04265 WP_000081550.1 732534..733526(-) (rpoS) [Escherichia coli strain RM19259]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGTGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715