Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DTOX_RS01300 Genome accession   NC_013216
Coordinates   281610..282983 (+) Length   457 a.a.
NCBI ID   WP_015755930.1    Uniprot ID   C8W3V5
Organism   Desulfofarcimen acetoxidans DSM 771     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 276610..287983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTOX_RS01280 (Dtox_0252) - 276823..277299 (+) 477 WP_015755926.1 CtsR family transcriptional regulator -
  DTOX_RS01285 (Dtox_0253) - 277313..277837 (+) 525 WP_015755927.1 UvrB/UvrC motif-containing protein -
  DTOX_RS01290 (Dtox_0254) - 277842..278903 (+) 1062 WP_015755928.1 protein arginine kinase -
  DTOX_RS01295 (Dtox_0255) - 279032..281464 (+) 2433 WP_015755929.1 ATP-dependent Clp protease ATP-binding subunit -
  DTOX_RS01300 (Dtox_0256) radA 281610..282983 (+) 1374 WP_015755930.1 DNA repair protein RadA Machinery gene
  DTOX_RS01305 (Dtox_0257) disA 282983..284062 (+) 1080 WP_015755931.1 DNA integrity scanning diadenylate cyclase DisA -
  DTOX_RS01310 (Dtox_0258) - 284097..284495 (-) 399 WP_015755932.1 hypothetical protein -
  DTOX_RS01315 (Dtox_0259) - 284699..285175 (+) 477 WP_015755933.1 CarD family transcriptional regulator -
  DTOX_RS01320 (Dtox_0260) - 285368..286519 (+) 1152 WP_015755934.1 PIN/TRAM domain-containing protein -
  DTOX_RS01325 (Dtox_0261) ispD 286506..287204 (+) 699 WP_015755935.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DTOX_RS01330 (Dtox_0262) ispF 287201..287674 (+) 474 WP_015755936.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49482.63 Da        Isoelectric Point: 7.5354

>NTDB_id=35277 DTOX_RS01300 WP_015755930.1 281610..282983(+) (radA) [Desulfofarcimen acetoxidans DSM 771]
MKQKTIYCCQECGSQSPRWVGRCPSCEAWNSMVEEMPVKSAGAVLRGKTEPVRQPVLLTEVLLYAENRIPTGIKEMDRVL
GGGIVPGSLILLGGEPGIGKSTLLLQVAFLIGKDNREVIYVCGEESMQQVRLRAERLNSISENVLLLAENNVDRVELQIK
ERKPFLVIIDSVQTIYKESLSSSPGSVGQVRECASQLMRLAKMMDTAIFLVGHVTKDGAIAGPKVLEHMVDAVLYFEGER
HQSFRLLRGVKNRFGSTNEIGIFNMAGNGLIEATNPSSLFMQHWQVPVPGSVIVPTIEGTRPLLVEIQALVCPTGFGLPR
RMATGVDYNRVALIIAVLERRLGYQLSKYDIYVNAVGGVKIEEPAVDLSIALAIASSFHDRPLGLGLAVAGEIGLTGEVR
PVASIEKRLSEAGELGFKKTIVPEANINPAIDCKSKAVGVKTVTEAVQVALQSGGIV

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=35277 DTOX_RS01300 WP_015755930.1 281610..282983(+) (radA) [Desulfofarcimen acetoxidans DSM 771]
ATGAAACAAAAGACCATCTATTGCTGCCAGGAATGTGGTAGTCAAAGCCCTCGTTGGGTAGGTCGCTGCCCGTCTTGTGA
GGCTTGGAATTCTATGGTGGAAGAGATGCCCGTTAAAAGTGCGGGTGCGGTTCTGCGGGGGAAAACAGAGCCTGTCAGGC
AGCCGGTTTTGTTAACAGAGGTACTGCTATATGCAGAAAATCGCATACCTACCGGTATTAAGGAAATGGATCGCGTATTG
GGTGGTGGCATTGTGCCGGGCTCTTTAATTTTATTGGGCGGTGAGCCTGGTATAGGCAAATCCACGCTTCTTTTGCAGGT
AGCTTTTTTAATAGGCAAAGATAATAGGGAAGTCATATATGTCTGTGGTGAGGAATCAATGCAGCAGGTTCGTTTGCGGG
CGGAGCGGCTTAATTCCATAAGCGAAAACGTATTGCTTTTAGCGGAAAATAATGTGGACCGGGTTGAATTGCAGATAAAA
GAGCGCAAGCCATTTTTGGTTATAATTGATTCGGTTCAGACAATATATAAAGAAAGCCTCTCTTCTTCTCCCGGCAGTGT
TGGTCAAGTTAGGGAGTGTGCCTCACAGCTAATGAGGCTGGCAAAAATGATGGACACAGCTATTTTTTTGGTCGGTCATG
TGACAAAGGACGGGGCAATTGCCGGTCCTAAAGTGCTTGAGCATATGGTAGATGCTGTTTTGTATTTTGAGGGAGAGAGG
CACCAGTCTTTCCGCTTGCTGAGAGGAGTTAAAAATCGTTTTGGTTCGACCAATGAAATAGGTATTTTTAATATGGCAGG
TAATGGTTTAATTGAAGCAACTAATCCCAGTTCTTTATTCATGCAGCATTGGCAGGTACCCGTACCCGGTTCAGTTATAG
TCCCTACGATAGAGGGGACTCGGCCTCTTCTGGTAGAGATTCAGGCATTGGTTTGTCCAACAGGTTTTGGTTTGCCCAGG
CGTATGGCAACAGGTGTCGACTATAATCGGGTGGCCTTAATCATAGCAGTTTTGGAGAGAAGATTAGGATACCAATTAAG
TAAGTATGATATTTATGTAAATGCTGTGGGCGGTGTGAAAATAGAGGAACCCGCGGTAGATTTGAGTATTGCTTTGGCCA
TAGCCTCCAGTTTTCATGATCGCCCATTGGGGTTGGGGTTGGCTGTGGCAGGTGAAATTGGCCTTACCGGGGAGGTCAGA
CCTGTAGCTTCTATAGAAAAAAGACTGTCTGAGGCCGGAGAGCTCGGATTTAAGAAAACTATAGTTCCTGAAGCTAATAT
AAATCCTGCCATTGATTGTAAATCAAAAGCAGTGGGAGTGAAAACTGTGACTGAGGCTGTTCAAGTAGCCTTGCAGTCTG
GGGGGATTGTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-218)

Subunit ChlI of Mg-chelatase (ChlI)

(337-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8W3V5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

51.762

99.344

0.514
  radA Streptococcus mitis NCTC 12261

51.77

98.906

0.512
  radA Streptococcus pneumoniae Rx1

51.77

98.906

0.512
  radA Streptococcus pneumoniae TIGR4

51.77

98.906

0.512
  radA Streptococcus pneumoniae D39

51.77

98.906

0.512
  radA Streptococcus pneumoniae R6

51.77

98.906

0.512
  radA Bacillus subtilis subsp. subtilis str. 168

51.101

99.344

0.508

Multiple sequence alignment