Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GFX43_RS15320 Genome accession   NZ_CP046448
Coordinates   2989276..2991105 (-) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZD01     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2984276..2996105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFX43_RS15285 (GFX43_015285) yjbM 2984738..2985373 (-) 636 WP_003245294.1 GTP diphosphokinase -
  GFX43_RS15290 (GFX43_015290) - 2985402..2985770 (-) 369 WP_003232922.1 hypothetical protein -
  GFX43_RS15295 (GFX43_015295) yjbK 2985895..2986467 (+) 573 WP_015383359.1 CYTH domain-containing protein -
  GFX43_RS15300 (GFX43_015300) cwlQ 2986482..2987207 (+) 726 WP_015383358.1 bifunctional muramidase/murein lytic transglycosylase -
  GFX43_RS15305 (GFX43_015305) bhbI 2987462..2987860 (+) 399 WP_015383357.1 thiol management oxidoreductase component -
  GFX43_RS15310 (GFX43_015310) spxH 2987857..2988756 (+) 900 WP_003245184.1 protease adaptor protein SpxH -
  GFX43_RS15315 (GFX43_015315) - 2989071..2989238 (+) 168 WP_003244944.1 hypothetical protein -
  GFX43_RS15320 (GFX43_015320) pepF 2989276..2991105 (-) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  GFX43_RS15325 (GFX43_015325) coiA 2991335..2992486 (-) 1152 WP_015483087.1 competence protein CoiA Machinery gene
  GFX43_RS15330 (GFX43_015330) - 2992530..2992644 (+) 115 Protein_3016 hypothetical protein -
  GFX43_RS15335 (GFX43_015335) mecA 2992647..2993303 (-) 657 WP_015383354.1 adaptor protein MecA Regulator
  GFX43_RS15340 (GFX43_015340) - 2993270..2993410 (-) 141 WP_119122854.1 hypothetical protein -
  GFX43_RS15345 (GFX43_015345) yjbE 2993580..2994236 (+) 657 WP_015483086.1 TerC family protein -
  GFX43_RS15350 (GFX43_015350) spx 2994279..2994674 (-) 396 WP_014476435.1 transcriptional regulator Spx -
  GFX43_RS15355 (GFX43_015355) yjbC 2994855..2995430 (-) 576 WP_015383352.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=352412 GFX43_RS15320 WP_003245178.1 2989276..2991105(-) (pepF) [Bacillus subtilis strain ZD01]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=352412 GFX43_RS15320 WP_003245178.1 2989276..2991105(-) (pepF) [Bacillus subtilis strain ZD01]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTGAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCGTCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCCACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCAGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTGAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCGGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTCGCACCATTAGGGGAAGAATATGCCTCAATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTAGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGTATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAGTATTTGCTGAACAATTTAGAAGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGCGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGTTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476