Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E4F35_RS12475 Genome accession   NZ_CP038224
Coordinates   2333050..2334426 (+) Length   458 a.a.
NCBI ID   WP_004193568.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain Yap2a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2328050..2339426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4F35_RS12450 (E4F35_12445) - 2328124..2329104 (+) 981 WP_004547886.1 DUF1853 family protein -
  E4F35_RS39130 - 2329068..2329286 (+) 219 WP_004535386.1 hypothetical protein -
  E4F35_RS12460 (E4F35_12455) thiD 2329530..2330339 (-) 810 WP_004534771.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  E4F35_RS12465 (E4F35_12460) lplT 2330450..2331757 (-) 1308 WP_004266648.1 lysophospholipid transporter LplT -
  E4F35_RS12470 (E4F35_12465) alr 2331969..2333039 (+) 1071 WP_004191260.1 alanine racemase -
  E4F35_RS12475 (E4F35_12470) radA 2333050..2334426 (+) 1377 WP_004193568.1 DNA repair protein RadA Machinery gene
  E4F35_RS12480 (E4F35_12475) - 2334590..2334856 (+) 267 WP_004534661.1 DUF2866 domain-containing protein -
  E4F35_RS12485 (E4F35_12480) - 2334944..2336884 (-) 1941 WP_004527300.1 ATP-binding cassette domain-containing protein -
  E4F35_RS12490 (E4F35_12485) - 2337030..2337509 (+) 480 WP_004192400.1 glutathione peroxidase -
  E4F35_RS12495 (E4F35_12490) cls 2337613..2339052 (-) 1440 WP_004191365.1 cardiolipin synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48744.18 Da        Isoelectric Point: 7.2044

>NTDB_id=352401 E4F35_RS12475 WP_004193568.1 2333050..2334426(+) (radA) [Burkholderia pseudomallei strain Yap2a]
MAKQKTVFVCTECGGQTPKWQGQCPSCHAWNTLVESVESAPSAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQ
ATIDAERPDVAVIDSIQTIYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVTQEGTRPLLVEVQALVDTANV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=352401 E4F35_RS12475 WP_004193568.1 2333050..2334426(+) (radA) [Burkholderia pseudomallei strain Yap2a]
ATGGCCAAGCAGAAGACGGTATTCGTCTGCACCGAGTGCGGCGGGCAGACGCCGAAGTGGCAGGGTCAATGCCCGTCGTG
CCACGCATGGAACACCCTCGTCGAATCGGTCGAGAGCGCGCCGTCCGCGCACCGCTTCCAGTCGCTCGCGAAGCGCGCGC
CCGTGCAGCGGCTCGCGGATATCGAGGCGGCCGACGTGCCGCGCTTCTCGACCGGGATCGGCGAATTCGACCGGGTGCTG
GGCGGCGGGCTTGTCGCGGGCGGCGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGTC
GCTCGCGCAGATCGCGAGCGAGCGGCCGGCGCTCTATATCAGCGGGGAGGAGTCGGGCGCGCAGATCGCGCTGCGCGCGC
AGCGGCTCGCGCTCCTCGAAGGCGGCGCGAGCGCGGCCGATCTGAAGCTGCTCGCCGAGATCCAGCTCGAGAAGATTCAG
GCGACGATCGACGCCGAGCGGCCCGACGTCGCGGTGATCGATTCGATCCAGACGATCTATTCGGAGGCGTTGACGTCGGC
GCCCGGTTCGGTCGCGCAAGTGCGCGAATGCGCGGCGCAATTGACGCGCATCGCGAAGCAATCGGGCACCGCGATCATCA
TGGTCGGCCACGTGACGAAAGAGGGCAACCTCGCCGGGCCGCGCGTGCTCGAGCACATCGTCGACACCGTGCTGTACTTC
GAAGGCGACACGCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTT
CGCGATGACCGAGCGCGGCCTGCGCGGCGTCGCGAATCCGTCCGCGCTGTTCCTGTCGCAGCACACGGAGGTCGTGCCGG
GCTCGTGCGTGCTCGTCACGCAGGAGGGCACGCGGCCGCTGCTCGTCGAGGTGCAGGCGCTCGTCGACACCGCGAACGTG
CCGAACCCGCGCCGGCTCGCGGTCGGCCTCGAGCAGAACCGGCTCGCGATGCTGCTCGCGGTGCTGCACCGGCACGCGGG
CATCGCGTGTTTCGACCAGGACGTGTTCCTGAACGCGGTCGGCGGCGTGAAGATCACCGAGCCGGCCGCCGATCTGGCGG
TGCTGCTCGCGATTCACTCGTCGATGCGTAACAAACCGTTGCCAAAGGGTCTGATCGTATTCGGCGAAGTCGGGCTCGCG
GGCGAGATCCGGCCGTCGCCGCGCGGGCAGGAACGCCTGCGCGAGGCCGCGAAGCTCGGCTTCACGACCGCGCTGATTCC
TAAGGCAAATGCGCCGAAACAGCCGATCGACGGGCTTCGCGTGCATGCGGTCGAGCGTATCGAACAGGCGATCGACCAGA
TTCGCGTGCTCGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(76-222)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(363-452)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.142

95.633

0.489
  radA Streptococcus mitis NCTC 12261

47.702

99.782

0.476
  radA Streptococcus pneumoniae D39

50

93.886

0.469
  radA Streptococcus pneumoniae TIGR4

50

93.886

0.469
  radA Streptococcus mitis SK321

50

93.886

0.469
  radA Streptococcus pneumoniae R6

50

93.886

0.469
  radA Streptococcus pneumoniae Rx1

50

93.886

0.469

Multiple sequence alignment